Academic Health Center Duluth
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Welcome to Duluth Medical Research Institute

The DMRI was established to link scientists from multiple preclinical, clinical, community, and population health sciences to forge innovative health research programs. The DMRI facilitates interactions among academics, clinical and population scientists, and community stakeholders to improve use and access to discoveries in medical sciences.

Our vision is to develop a nationally respected medical research institute, building on the University of Minnesota's record of excellence in biomedical and clinical research and on existing areas of expertise, while attracting outstanding multidisciplinary scientists to conduct innovative translational research.

For more information, please contact us at or at the following Address

Duluth Medical Research Institute
Medical School Building
University of Minnesota Medical School
1035 University Drive
Duluth, MN 55812, U.S.A.
(218) 726-8894





Dr. Largaespada

DMRI Seminar Photo Dr. David Largaespada

“Cancer Gene Discovery and Subclone Lineage Tracking Using Transposable Elements in Mouse Model”

April 21st, 2014 12:00 – 1:00 SMED 142

Abstract: Transposons are being developed as tools for gene transfer and insertional mutagenesis in vertebrate model organisms. The Sleeping Beauty (SB) transposon system is one such tool. Our data indicate that the SB system is ideally suited to forward genetic screens for cancer genes in mouse somatic cells. The system requires creating mice that harbor both a transposon array of the insertionally mutagenic SB vector, T2/Onc, and express the transposase enzyme in the target somatic tissue. If transposition can induce cancer, then tumor DNA is studied by cloning insertion sites. These insertion sites are analyzed and T2/Onc insertions at reproducibly mutated genes are sought. Results from ubiquitous and tissue-specific transposon mutagenesis for cancer gene discovery will be presented. We have recently completed screens for gastrointestinal tract cancer, hepatocellular carcinoma, medulloblastoma, metastatic osteosarcoma, and malignant peripheral nerve sheath tumors. Methods for discovery of cooperating cancer mutations and lineage tracking of metastatic disease using SB-accelerated models of cancer will be presented. Finally, data will be shown on the use of SB for somatic cell gene transfer as an approach for cancer gene validation.

Biography: Dr. Largaespada is currently a Full Professor in the Department of Genetics, Cell Biology and Development and the Department of Pediatrics at the University of Minnesota. He is the co-leader of the Genetic Mechanisms of Cancer Program and the Associate Director for Basic Research in the Masonic Cancer Center at University of Minnesota. Dr. Largaespada currently holds the Margaret Harvey Schering Land Grant Chair in Cancer Genetics. Dr. Largaespada was awarded the McKnight Land-Grant Professorship in 2000 and is an American Cancer Society Scholar. He has published over 126 scientific articles and teaches courses in the molecular biology of cancer and mammalian gene transfer.




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