Contact Information Phone: 218-726-8345 Fax: 218-726-8014 Email: eperkins@d.umn.edu Address: 249 SMed 1035 Univeristy Dr. Duluth, MN 55812 http://www.med.umn.edu/duluth

Assistant Professor

Links

Research

An additional area of research involves the genetic analysis of the human cell cycle regulatory gene cyclin A1 and its role in the early stages of cancer, particularly myeloid leukemias. Given the apparent evolutionary conservation of cell cycle control mechanisms between human and microbial systems, we are utilizing a yeast-based genetic assay to functionally characterize human cyclin A1 and identify downstream targets and regulators of cyclin A1 activity.

Education

Laboratory Personnel

Publications

Publications on PubMed

Selected Recent Publications (including submitted)
Perkins, E., Wood, V., Sterling, J., Hashem, V., Resnick, M. A. (2004) Functional analysis of human cyclin A1 in Saccharomyces cerevisiae and its impact on genes affecting DNA metabolism. (submitted).

Lindenbaum, M., Perkins, E. Csonka, E., Fleming, E. Garcia, L., Greene, A., L., Hadlaczky, G., Lee, E., Leung, J., MacDonald, N., Maxwell, A., Mills, K., Monteith, D., Perez, C., Shellard, J., Stewart, S., Stodola, T., Vanderbyl, S., Ledebur, H. (2004) A mammalian artificial chromosome engineering system (ACE System) applicable to biopharmaceutical protein production, transgenesis and gene-based cell therapy. (submitted).

Vanderbyl, S., MacDonald, N., Sidhu, S., Gung, L., Telenius, A., Perez, C., Perkins, E. (2004) Transfer and stable transgene expression of a mammalian artificial chromosome into bone marrow derived human mesenchymal stem cells. Stem Cells. 22: 324-333.

Stewart, S., MacDonald, N., Perkins, E., deJong, G., Perez, C. Lindenbaum, M. (2002) Retrofitting of a satellite repeat DNA-based murine artificial chromosome (ACes) to contain loxP recombination sites. Gene Ther. 9: 719-723.

Perkins, E., Sun D., Nguyen, A., Tulac, S., Francesco, M., Tavana, H., Nguyen, H., Tugendreich, S., Barthmaier, P., Couto, J., Yeh, E., Thode, S., Jarnagin, K., Jain, A., Morgans, D., Melese, T. (2001) Novel inhibitors of poly(ADP-ribose) polymerase/ PARP1 and PARP2 identified using a cell-based screen in yeast. Cancer Res. 61: 4175-4183.

Tugendreich, S., Perkins, E., Couto, J., Barthmaier, P., Sun, D., Tang, S., Tulac, S., Nguyen, A., Yeh, E., Mays, A., Wallace, E., Lila, T., Shivak, D., Prichard, M., Andrejka, L., Kim, R., Melese, T. (2001) A streamlined process to phenotypically profile heterologous cDNAs in parallel using yeast cell-based assays. Genome Res. 11: 1899 - 1912.

Perkins, E., Sterling, J., Hashem, V., Resnick, M. A. (1999) Yeast and human genes that affect the Escherichia coli SOS response. Proc. Natl. Acad. Sci. USA 96: 2204-2209.

PATENTS: 3 Issued and 12 Filed
Patent number: Filed. Citation: de Jong, G., Perkins, E., Telenius, A., MacDonald, N. Methods for deliveing nucleic acid molecules into cells and assessment thereof. Filed December 4, 2003. (Application No. 20030224522)

Patent number: Filed. Citation: Perkins, E., Perez, C., Lindenbaum, M., Greene, A., Leung, J., Fleming, E., Stewart, S. Chromosome-based platforms. Filed June 26, 2003. (Application No. 20030119104)

Patent number: Filed. Citation: Melese, T., Perkins, E. L., Nguyen, A. T. Q., Sun, D. Modulators of phosphoinositide 3-kinase. Filed August 7, 2003. (Application No. 20030149074)

Patent number: Filed. Citation: Melese, T., Perkins, E. L., Nguyen, A. T. Q., Sun, D. Modulators of phosphoinositide 3-kinase. Filed August 21, 2003. (Application No. 20030158212)

Patent number: Filed. Citation: Sun, D., Perkins, E. L., Tugendreich, S., Modulators of RhoC activity. Filed September 11, 2003. (Application No. 20030171341)

Patent number: Filed. Citation: Sun, D., Perkins, E. L., Tugendreich, S., Modulators of RhoC activity. Filed September 11, 2003. (Application No. 20030171387)

Patent number: Filed. Citation: Sun, D., Perkins, E. L., Tugendreich, S., Lee, V. J. Modulators of RhoC activity. Filed September 11, 2003. (Application No. 20030171390)

Patent number: Filed. Citation: Sun, D., Perkins, E. L., Tugendreich, S., Lee, V. J. Modulators of RhoC activity. Filed September 18, 2003. (Application No. 20030176474)

Patent number: Filed. Citation: Melese, T., Perkins, E. L., Tugendreich, S. Method for generating a pathway reporter system. Filed January 10, 2002. (Application No. 20020004197)

Patent number: Filed. Citation: Perkins, E. L. and Tugendreich, S. M. Vector construction by host cell-mediated recombination. Filed October 17, 2002 (Application No. 20020151058)

Patent number: Filed. Citation: Fabijanski, S., Perkins, E., Perez, C. Plant artificial chromosome, uses therof and methods of preparing plant artificial chromosomes. Filed May 5, 2001. (Application Serial No. 60/294687)

Patent number: Filed. Citation: Perkins, E., Perez, C., Lindenbaum, M. Greene, A., Leung, J. Fleming, E. Stewart, S. Shellard, J. Chromosome-based platforms. Filed May 30, 2001. (Application No. 20030119104)

Patent number: 6,391,642. Citation: Resnick, M.A. Larionov, V., Kouprina, N., Perkins, E. Transformation-associated recombination cloning.

Patent number: 5,489,524. Citation: Resnick, M. A., Chow, T., Perkins, E. Chimeric protein that has a human Rho motif and deoxyribonuclease activity (CIP). February 6, 1996.

Patent number: 5,324,830. Citation: Resnick, M. A., Chow, T., Perkins, E. Chimeric protein that has a human Rho motif and deoxyribonuclease activity. June 28, 1994.

Recent Presentations
Vanderbyl, S., MacDonald, N., de Jong, G., Perkins, E., Perez, C. (2002) High efficiency transfer of an artificial chromosome into postnatal human mesenchymal stem cells. Keystone Symposia, Stem Cells: Origins, Fates and Functions. Keystone, CO. [Poster]

Vanderbyl, S., MacDonald, N., de Jong, G., Perkins, E., Perez, C. (2002) Flow cytometric techniques allow for high efficiency transfer of an artificial chromosome into a variety of mammalian primary cell lines, improving potential for use in gene therapy applications. XXI Congress of the International Society for Analytical Cytology. San Diego, CA. [Poster]

Telenius, A., Macdonald, N., Vanderbyl, S., de Jong, G., Perkins, E., (2002) Enhanced Delivery of Artificial Chromosomes into Immortalized and Primary Cells by Cell Cycle Synchronization. American Society of Gene Therapy Fifth Annual Meeting. Boston, MA. [Poster]

Vanderbyl, S., MacDonald, N., de Jong, G., Sidhu, S., Gung, L., Perez, C., Perkins, E. (2002) Efficient transfer of an artificial chromosome platform into adult human mesenchymal stem cells. American Society of Gene Therapy Fifth Annual Meeting. Boston, MA. [Poster]

Vanderbyl, S., MacDonald, N., Sidhu, S., Gung, L., Telenius, A., de Jong, G., Perez, C., Perkins, E. (2002) Differentiation of adult derived human mesenchymal stem cells genetically modified with an artificial chromosome platform. 2nd Annual Mesenchymal & Nonhematopoietic Stem Cell Conference. New Orleans, LA [Poster]

Perkins, E. (2001) An efficient, tractable chromosome-based platform for gene therapy. American Society of Gene Therapy Fourth Annual Meeting. Seattle, WA [Invited Speaker]

Perkins, E. (2001) Development and application of Artificial Chromosome expression systems (ACes). 2001 Congress on In Vitro Biology. St. Louis, MO [Invited Speaker]

Perkins, E. (2001) An efficient, tractable chromosome-based platform for gene therapy. BioFuture 2001. Toronto, ONT [Invited Speaker]

Perkins, E. (2001) Development of chromosome-based expression platforms for therapeutic protein production and human gene therapy. Fifth Gene Delivery and Cellular Protein Expression Conference. Semmering near Vienna, Aus. [Invited Speaker]

Perkins, E. (2000) Yeast technology platform for postgenomic research. Cambridge Healthtech Institute Third Annual Meeting, Protein Expression and Protein Production. Arlington, VA [Invited Speaker]