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Contact Information
| Phone: | 218-726-7502 |
| Fax: | 218-726-8014 |
| Email: | jprohask@d.umn.edu |
Address:
259 SMed
1035 Univeristy Dr.
Duluth, MN 55812 |
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Professor
Links
Academic Health Center Graduate Programs
Integrated Biosciences Graduate Program
TEMA
Dr. Prohaska's Nutrition Website
Research
The focus of my research program is to understand the biochemical functions of copper and consequences of imbalances of this essential metal due to environmental or genetic factors. The laboratory's primary research approach involves nutritional biochemistry and molecular biology. My current research interests include:
1. Investigation of the impact of dietary copper deficiency during early development on the central nervous system is under study. Characterization of the impact of low intracellular copper on the expression of proteins that function as known cuproenzymes and copper transporters is being investigated to test hypotheses relating biochemistry to behavior.
2. Evaluation of the cuproenzyme peptidylglycine alpha-amidating monooxygenase (PAM) and the copper chaperone for superoxide dismutase (CCS) as potential molecular markers to assess nutritional copper status in humans is currently under investigation.
My lab is currently funded by National Institute of Child Health and Human Development and the USDA National Research Initiative Bioactive Food Components for Optimal Health Programs.
Ph.D. Graduate opportunities in my lab may be pursued through University of Minnesota programs in Biochemistry, Molecular Biology and Biophysics, Integrated Biosciences, Nutrition, or Toxicology.
Education
Ph.D. Michigan State University, Biochemistry, 1974
Laboratory Personnel
Margaret Broderius, Assistant Scientist
Anya Gybina, Graduate Student
Kyle Nelson, Graduate Student
Josh Pyatskowit, Graduate Student
Jacob (Jack) Lyons, Graduate Student
Publications
Publications on PubMed
Peer-Reviewed Manuscripts - Past Five Years
Pyatskowit, J.W. and Prohaska, J.R. (2008) Multiple mechanisms account for lower plasma iron in young copper deficient rats. Biometals 21, 343-352.
Gybina, A.A. and Prohaska, J.R. (2008) Copper deficiency results in AMP-activated protein kinase activation and acetylCoA carboxylase phosphorylation in rat cerebellum. Brain Res. 1204, 69-76.
Suazo, M., Olivares , F., Mendez, M.A., Pulgar, R., Prohaska, J.R., Arrendondo, M., Pizarro, F., Olivares, M., Araya, M., and González, M. (2008) CCS and SOD1 mRNA are reduced after copper supplementation in peripheral mononuclear cells of individuals with high serum ceruloplasmin concentration. J. Nutr. Biochem. 19, 269-274.
Pyatskowit, J.W. and Prohaska, J.R. (2008) Copper deficient rats and mice both develop anemia but only rats have lower plasma and brain iron levels. Comp. Biochem. Physiol. C. 147, 316-323.
Pyatskowit, J.W. and Prohaska, J.R. (2007) Rodent brain and heart catecholamine levels are altered by different models of copper deficiency. Comp. Biochem. Physiol. C. 145, 275-281.
Gybina, A., and Prohaska, J.R. (2006) Variable response of selected cuproproteins in rat choroid plexus and cerebellum following perinatal copper deficiency. Genes and Nutrition 1, 51-60.
Prohaska, J.R., and Broderius, M. (2006) Plasma peptidylglycine alpha-amidating monooxygenase (PAM) and ceruloplasmin are affected by age and copper-status in rats and mice. Comp. Biochem. Physiol. B. 143, 360-366.
Kuo, Y-M., Gybina, A.A., Pyatskowit, J.W., Gitschier, J., and Prohaska, J.R. (2006) Copper transport protein (Ctr1) levels in mice are tissue specific and dependent on copper status. J. Nutr. 136, 21-26.
Pyatskowit, J.W., and Prohaska, J.R. (2005) L-threo 3,4-dihydroxyphenylserine treatment during mouse perinatal and rat postnatal development does not alter the impact of dietary copper deficiency. Nutr. Neurosci. 8, 173-181.
Prohaska, J.R., and Gybina, A.A. (2005) Rat brain iron concentration is lower following perinatal copper deficiency. J. Neurochem. 93, 698-705.
Prohaska, J.R., Gybina, A.A., Broderius, M., and Brokate, B. (2005) Peptidylglycine alpha-amidating monooxygenase activity and protein are lower in copper-deficient rats and suckling copper-deficient mice. Arch. Biochem. Biophys. 434, 212-220.
Prohaska, J.R., and Gybina, A.A. (2004) Intracellular copper transport in mammals. J. Nutr. 134, 1003-1006.
West, E.C., and Prohaska, J.R. (2004) Cu,Zn-superoxide dismutase is lower and copper chaperone CCS is higher in erythrocytes of copper-deficient rats and mice. Exp. Biol. Med. 229, 756-764.
Penland, J., and Prohaska, J.R. (2004) Abnormal motor function persists following recovery from perinatal copper deficiency in rats. J. Nutr. 134, 1984-1988.
Chung, J., Prohaska, J.R., and Wessling-Resnick, M. (2004) Ferroportin-1 mRNA is not upregulated in copper-deficient mice. J. Nutr. 134, 517-521.
Gybina, A., and Prohaska, J.R. (2003) Increased rat brain cytochrome c correlates with degree of perinatal copper deficiency rather than apoptosis. J. Nutr. 133: 3361-3368.
Prohaska, J.R., Broderius, M., and Brokate, B (2003) Metallochaperone for Cu,Zn-Superoxide Dismutase (CCS ) Protein but not mRNA is Higher in Organs from Copper-Deficient Mice and Rats. Arch. Biochem. Biophys. 417: 227-234.
Prohaska, J.R., Geissler, J., Brokate, B., and Broderius, M. (2003) Cu,Zn-superoxide dismutase protein but not mRNA is lower in Cu-deficient mice and mice lacking the metallochaperone CCS. Exp. Biol. Med. 228: 959-966.
Hamza, I., Prohaska, J., and Gitlin, J. D. (2003) Essential role for atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase. Proc. Natl. Acad. Sci. USA 100: 1215-1220.
Published Abstracts - Past Five Years
Prohaska, J.R., and Walters, M. (2008) Copper-deficient rats exhibit no alterations in plasma or brain NO metabolites, 3-nitrotyrosine peptide abundance, or NOS mRNA expression. FASEB J. 22, 443.1.
Gybina, A., and Prohaska, J.R. (2007) Cerebella of young copper deficient rats have increased AMPK phosphorylation but decreased fructose 2,6 bisphosphate levels. FASEB J. 21, A723.
Pyatskowit, J.W., Nelson, K.T., Gybina, A.A. and Prohaska, J.R.(2007) Suckling copper-deficient mice and rats exhibit opposite changes in plasma iron, different impacts on brain iron concentration, but both exhibit severe anemia. FASEB J. 21, A163.
Prohaska, J.R. (2006) Impact of copper-status and age on rodent plasma peptidylglycine alpha-amidating monooxygenase and ceruloplasmin. FASEB J. 20, A1066.
Pyatskowit, J.W., and Prohaska, J.R. (2006) Copper-status and age alter suckling rodent brain and heart catecholamine levels. FASEB J. 20, A1066.
Kuo, Y.M., Gybina, A.A., Gitschier, J. and Prohaska, J.R. (2006) Copper transporter (Ctr1) expression in mouse tissue is impacted by age and dietary copper. FASEB J. 20, A553.
Prohaska, J.R., and Gybina, A.A. (2005) Rat brain iron accumulation is dependent on adequate dietary copper FASEB J. 19, A983.
Pyatskowit, J.W., and Prohaska, J.R. (2005) L-threo 3,4-dihydroxyphenylserine (DOPS) treatment during perinatal development does not alter the impact of dietary copper deficiency in mice. The Toxicologist 84, A279.
Chung, J., Prohaska J. R., and Wessling-Resnick, M. (2004) Copper deficiency in mice increases hepatic iron stores but does not up-regulate expression of the iron exporter Ferroportin-1. FASEB J. 18, A484.
Prohaska, J.R., and West, E. (2004) Erythrocytes from Copper-Deficient Mice and Rats have Lower Cu,Zn-superoxide dismutase (SOD) but Higher Copper Chaperone (CCS) Levels. FASEB J. 18, A915.
Gybina, A., and Prohaska, J.R. (2004) Elevated Rodent Brain Cytochrome C is Correlated with Degree of Copper Deficiency not Apoptosis. FASEB J. 18, A483.
Prohaska, J.R., Geissler, J., Broderius, M. and Brokate, B. (2003) Copper-deficient mice and rats have lower Cu,Zn-superoxide dismutase activity and protein but higher metallochaperone CCS levels. FASEB J. 17, A378.
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