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Shared Resources
The mission of the Center for Immunology at the University of Minnesota is to coordinate and promote efforts in immunology research, education and clinical applications. The shared resources described below proved a stable, cost-effective and technologically relevant means of supporting those goals and are available to other researchers both within and outside the University community. Location: Medical Bioscience Building (MBB)
Contact: Deb Lins, 612-626-2476 RS 2000 X-Ray Irradiator
The RS 2000 is the non-isotope alternative to radioactive source equipment for research irradiation applications. This system is powerful and flexible enough to assist with biological studies in cancer, bio-technology and selected institutional work including: * Human Genome * Myeloablation * Stem Cell Research * Small animal work * Feeder cell production The RS 2000 has user-variable dose rates up to 6 Gy per minute whereas a gamma irradiator's dose rate is dependent on the isotope used and the remaining curies encapsulated. Rad Source has developed the expertise to produce a dose equivalency to gamma irradiators. The RS 2000 allows for one cycle irradiation of animals in a standard filtered Allentown Cage, which is provided with the equipment and provides 95% horizontal dose uniformity within the rearing cage using our patented reflective holder Flow Cytometry Contact Person: Paul Champoux, 612-626-3068
An important asset to the Center is our Flow Cytometry Core Facility. Consisting of 5 benchtop analyzers, a dual G5 Mac workstation and high-quality color printing capability. Instrumentation consists of 1 FACScan, capable of 5-parameter acquisition, and 2 FACSCaliburs, capable of 6 parameter acquisition and 2 Becton-Dickinson LSR II's. One FACSCalibur(E1764) is equipped with the Autoloader and Sort module. LSR II(H1160) is equipped with 4 lasers(14 parameter including UV) while the other LSR II(H1010) has 3 lasers(11 parameter) including a 50mW Violet. Instrumentation
A variety of common laboratory instrumentation is also shared by the center staff members. A sampling of this equipment encompasses centrifugation, spectrophotometry, real-time PCR, radioisotope counting, and protein purification.
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