Yoji Shimizu, Ph.D.

Professor
612-626-6849
shimi002@umn.edu

Educational Background

• Cornell University, Ithaca, NY (1982), B.A. (Biological Sciences)
• University of Wisconsin, Madison, WI (1987), Ph.D. (Genetics)
• National Institutes of Health, Bethesda, MD (1990), Postdoctoral Training (Immunology)

Professional Background

• Director of Graduate Studies, University of Minnesota Medical School, Minneapolis, MN Microbiology, Immunology & Cancer Biology (MICaB) Ph.D. Graduate Program, 2004 - present
• Professor, University of Minnesota Medical School, Minneapolis, MN, Department of Laboratory Medicine and Pathology, 1999 - present
• Immunology program leader, University of Minnesota Cancer Center, Minneapolis, MN, 1996 - present
• Associate Professor, University of Minnesota Medical School, Minneapolis, MN, Department of Laboratory Medicine and Pathology, 1994 - 1999
• Harry Kay Chair in Biomedical Research, University of Minnesota Medical School, Minneapolis, MN, Department of Laboratory Medicine and Pathology, 1994 - present
• Assistant Professor , University of Michigan Medical School, Ann Arbor, MI, Department of Microbiology and Immunology, 1991 - 1994
• Damon Runyon-Walter Winchell Cancer Research Fund Postdoctoral Fellow in the laboratory of Dr. Stephen Shaw, Experimental Immunology Branch, NIH/NCI, Bethesda, MD, 1988 - 1990
• Intramural Research Training Award (IRTA) Fellow in the laboratory of Dr. Stephen Shaw, Experimental Immunology Branch, NIH/NCI, Bethesda, MD, 1987 - 1988
• NIH Predoctoral Trainee in the laboratory of Dr. Robert DeMars, Laboratory of Genetics, University of Wisconsin, Madison, WI, 1982 - 1987

Professional Honors

• Elected to The Phi Beta Kappa Society, Theta of New York , Cornell University, 1981
• Graduated with distinction in all subjects, Cornell University, 1982
• Recipient of two year Postdoctoral Fellowship, American Cancer Society, Declined in favor of Damon Runyon Fellowship, 1988
• Recipient of Postdoctoral Fellowship for Basic Scientists (DRG-993), Damon Runyon-Walter Winchell Cancer Research Fund, 1988 - 1990
• Recipient of Junior Faculty Research Award, American Cancer Society, 1991
• Special Reviewer, Pathobiochemistry Study Section, February 1993, NIH, 1993
• Guest Reviewer, Pathology A Study Section, June 1995, NIH, 1995
• Member, Pathobiochemistry Study Section, NIH, 1995 - 1999
• Section Editor, Journal of Immunology, 1996 – 2001
• Chair, Autumn Immunology Conference, 1999
• Highly cited researcher in immunology, ISIHighlyCited.com, 2003

Publications

• Shimizu, Y., Koller, B., Geraghty, D., Orr, H., Shaw, S., Kavathas, P. and DeMars, R.: Transfer of cloned human class I major histocompatibility complex genes into HLA mutant human lymphoblastoid cells. Mol. Cell. Biol. 1986; 6:1074-1087.
• Shimizu, Y., Geraghty, D.E., Koller, B.H., Orr, H.T. and DeMars, R.: Transfer and expression of three cloned human non-A,B,C class I histocompatibility complex genes in mutant lymphoblastoid cells. Proc. Natl. Acad. Sci. USA 1988; 85:227-231.
• Shimizu, Y., van Seventer, G.A., Horgan, K.J. and Shaw, S.: Roles of adhesion molecules in T cell recognition: fundamental similarities between four integrins on resting human T cells (LFA-1, VLA-4, VLA-5, VLA-6) in expression, binding, and costimulation. Immunol. Rev. 1990; 114:109-143.
• Shimizu, Y., van Seventer, G.A., Horgan, K.J. and Shaw, S.: Regulated expression and binding of three VLA (beta1) integrin receptors on T cells. Nature 1990; 345:250-253.
• Webb, D.S.A., Shimizu, Y., van Seventer, G.A., Shaw, S. and Gerrard, T.L.: LFA-3, CD44, and CD45: physiologic triggers of human monocyte TNF and IL-1 release. Science 1990; 249:1295-1297.
• Shimizu, Y., Shaw, S., Graber, N., Gopal, T.V., Horgan, K.J., van Seventer, G.A. and Newman, W.: Activation-independent binding of human memory T cells to adhesion molecule ELAM-1. Nature 1991; 349:799-802.
• Shimizu, Y., Newman, W., Graber, N., Horgan, K.J., Beall, L.D., Gopal, T.V., van Seventer, G.A. and Shaw, S.: Four molecular pathways of T cell adhesion to endothelial cells: roles of LFA-1, VCAM-1 and ELAM-1 and changes in pathway hierarchy under different activation conditions. J. Cell Biol. 1991; 113:1203-1212.
• Shimizu, Y. and Shaw, S.: Lymphocyte interactions with extracellular matrix. FASEB J. 1991; 5:2292-2299.
• Shimizu, Y., van Seventer, G.A., Ennis, E., Newman, W., Horgan, K.J. and Shaw, S.: Crosslinking of the T cell-specific accessory molecules CD7 and CD28 modulates T cell adhesion. J. Exp. Med. 1992; 175:577-582.
• Shimizu, Y., Newman, W., Tanaka, Y. and Shaw, S.: Lymphocyte interactions with endothelial cells. Immunol. Today. 1992; 13:106-112.
• Ennis, E., Isberg, R.R. and Shimizu, Y.: VLA-4-dependent adhesion and costimulation of resting human T cells by the beta1 bacterial ligand invasin. J. Exp. Med. 1993; 177:207-212.
• Mobley, J.L., Reynolds, P.J. and Shimizu, Y.: Regulatory mechanisms underlying T cell integrin receptor function. Semin. Immunol. 1993; 5:227-236.
• Shimizu, Y. and Mobley, J.L.: Distinct divalent cation requirements for integrin-mediated CD4+ T lymphocyte adhesion to ICAM-1, fibronectin, VCAM-1, and invasin. J. Immunol. 1993; 151:4106-4115.
• Shimizu, Y. and Shaw, S.: Mucins in the mainstream (News & Views). Nature 1993; 366: 630-631.
• Shimizu, Y. (ed.) Lymphocyte adhesion molecules, R.G. Landes Co., Austin, 1993.
• Mobley, J.L. and Shimizu, Y.: Measurement of cellular adhesion under static conditions. in Coligan, J.E., Kruisbeek, A.M., Margulies, D.H., Shevach, E.M. and Strober, W. (eds.) Current Protocols in Immunology, 1994.
• Shimizu, Y.: Signaling to and from T cell integrins. in Takada, Y. (ed.) Integrins: the biological problems, CRC Press, Boca Raton, 1994.
• Mobley, J.L., Ennis, E. and Shimizu, Y.: Differential activation-dependent regulation of integrin function on human leukemic T cell lines. Blood 1994; 83:1039-1050.
• Shimizu, Y., Finkelstein, L.D. and Chan, A.S.H.: A role for phosphatidylinositol 3-kinase in the regulation of beta1 integrin activity by the CD2 antigen. J. Cell Biol. 1995; 131:1867-1880.
• Mobley, J.L., Ennis, E. and Shimizu, Y.: Isolation and characterization of cell lines with genetically distinct mutations downstream of protein kinase C that result in defective activation-dependent regulation of T cell integrin function. J. Immunol. 1996; 156:948-956.
• Mobley, J.L., Ennis, E. and Shimizu, Y.: Isolation and characterization of cell lines with genetically distinct mutations downstream of protein kinase C that result in defective activation-dependent regulation of T cell integrin function. J. Immunol. 1996; 156:948-956.
• Zell, T., Hunt, S.W., III, Mobley, J.L., Finkelstein, L.D. and Shimizu, Y.: CD28-mediated upregulation of beta1 integrin adhesion involves phosphatidylinositol 3-kinase. J. Immunol. 1996;156:883-886.
• Hunt, S.W., III, Kellermann, S.-A. and Shimizu, Y.: Integrins, integrin regulators and the extracellular matrix: the role of signal transduction in leukocyte migration. in Bochner, B.S. (ed.) Adhesion molecules in allergic disease, Marcel Dekker, New York, 1997.
• Shimizu, Y. and Hunt, S.W., III: Regulating integrin-mediated adhesion: one more function for PI 3-kinase? Immunol. Today 1996; 17:565-573.
• Chan, A.S.H., Mobley, J.L., Fields, G.B. and Shimizu, Y.: CD7-mediated regulation of integrin adhesiveness on resting human T cells involves tyrosine phosphorylation-dependent activation of phosphatidylinositol 3-kinase. J. Immunol. 1997; 159:934-942.
• Hunter, A.J. and Shimizu, Y.: alpha4beta1 integrin-mediated tyrosine phosphorylation in T cells: characterization of Crk- and Fyn-associated substrates (pp105, pp115, HEF1) and integrin-dependent activation of p59fyn. J. Immunol. 1997; 159:4806-4814.
• Finkelstein, L.D., Reynolds, P.J., Hunt, S.W., III and Shimizu, Y.: Structural requirements for beta1 integrin-mediated tyrosine phosphorylation in human T cells. J. Immunol. 1997; 159:5355-5363.
• Kivens, W.J., Hunt, S.W., III, Mobley, J.L., Zell, T., Dell, C.L., Bierer, B.E. and Shimizu, Y.: Identification of a proline-rich sequence in the CD2 cytoplasmic domain critical for regulation of integrin-mediated adhesion and activation of phosphatidylinositol 3-kinase. Mol. Cell. Biol. 1998; 18:5291-5307.
• Zell, T., Warden, C.S., Cook, M.E., Dell, C.L., Hunt, S.W., III and Shimizu, Y.: Regulation of beta1 integrin-mediated adhesion by the Cbl adapter protein. Curr. Biol. 1998; 8:814-822.
• Romzek, N.C, Harris, E.S., Dell, C.L., Skronek, J., Hasse, E., Reynolds, P.J., Hunt, S.W., III and Shimizu, Y.: Use of a beta1 integrin-deficient human T cell to identify beta1 integrin cytoplasmic domain sequences critical for integrin function. Mol. Biol. Cell 1998; 9:2715-2727.
• Chen, C., Mobley, J.L., Dwir, O., Shimron, F., Grabovsky, V., Lobb, R.R., Shimizu, Y. and Alon, R.: High affinity very late antigen-4 subsets expressed on T cells are mandatory for spontaneous adhesion strengthening but not for rolling on VCAM-1 in shear flow. J. Immunol. 1999; 162:1084-1095.
• Shimizu, Y., Rose, D.M. and Ginsberg, M.H.: Integrins in the immune system. Adv. Immunol. 1999; 72:325-380.
• Finkelstein, L.D. and Shimizu, Y.: The role of phosphoinositide 3-OH kinase and the cbl adaptor protein in coupling the alpha4beta1 integrin to mitogen activated protein kinase signaling.  Biochem. J. 2000; 345:385-392.
• Adelsman, M.A., McCarthy, J.B. and Shimizu, Y.: Stimulation of beta1 integrin function by epidermal growth factor and heregulinb has distinct requirements for erbB2 but a similar dependence on phosphoinositide 3-OH kinase. Mol. Biol. Cell 1999; 10:2861-2878.
• Woods, M.L., Cabañas,C. and Shimizu, Y. (2000) Activation-dependent changes in soluble fibronectin binding and expression of beta1 integrin activation epitopes in T cells: relationship to T cell adhesion and migration. Eur J. Immunol. 30: 38-49.
• Zell, T., Kivens, W.J., Kellermann, S.-A. and Shimizu, Y. (1999) Regulation of integrin function by T cell activation: points of convergence and divergence. Immunol. Res. 20: 127-145.
• Finkelstein, L.D. and Shimizu, Y.: The role of phosphoinositide 3-OH kinase and the cbl adaptor protein in coupling the alpha4beta1 integrin to mitogen activated protein kinase signaling. Biochem. J. 2000; 345:385-392.
• Epler, J.A., Liu, R. and Shimizu, Y.: From the ECM to the cytoskeleton and back: how integrins orchestrate T cell action. Dev. Immunol. 2000; 7:155-170. 
• Hunter, A.J., Ottoson, N., Boerth, N., Koretzky, G.A. and Shimizu, Y.: Cutting edge: a novel function for the SLAP-130 adapter protein in beta1 integrin signaling and T lymphocyte migration. J. Immunol. 2000; 164:1143-1147
• Epler, J.A., Liu, R., Chung, H., Ottoson, N.C. and Shimizu, Y.: Regulation of beta1 integrin-mediated adhesion by T cell receptor signaling involves ZAP-70 but differs from signaling events that regulate transcriptional activity. J. Immunol. 2000; 165:4941-4949
• Shimizu, Y.: The choreography of signaling at the plasma membrane. Immunol. Today 2000; 21:413
• Woods, M.L., Kivens, W.J., Adelsman, M.A., Qiu, Y., August, A. and Shimizu, Y.:  A novel function for the Tec family tyrosine kinase Itk in activation of b1 integrins by the T cell receptor.  EMBO J. 2001; 20:1232-1244.
• Peterson, E.J., Woods, M.L., Dmowski, S.A., Derimanov, G. Jordan, M.S., Wu, J.N., Myung, P.S., Liu, Q.-H., Pribila, J.T., Freedman, B.S., Shimizu, Y. and Koretzky, G.A.: Coupling of the TCR to integrin activation by SLAP-130/Fyb.  Science 2001; 293:2263-2265.
• Woods, M.L. and Shimizu, Y.:  Signaling networks regulating b1 integrin-mediated adhesion of T lymphocytes to the extracellular matrix.  J. Leukoc. Biol. 2001; 69:874-880.
• Chen, R., Kim, O., Li, M., Ilic, D., Xiong, X., Guan, J.-L., Kung, H.-J., Chen, H., Damsky, C., Shimizu, Y. and Qiu, Y.:  Regulation of a PH domain-containing tyrosine kinase Etk by focal adhesion kinase through the FERM domain.  Nat. Cell Biol. 2001; 3:439-444.
• Ottoson, N.C., Pribila, J.T., Chan, A.S.H. and Shimizu, Y.: Cutting edge: T cell migration regulated by CXCR4 chemokine receptor signaling to ZAP-70 tyrosine kinase. J. Immunol. 2001; 167:1857-1861.
• Kellermann, S.-A., Dell, C.L., Hunt, S.W., III and Shimizu, Y.: Genetic analysis of integrin activation in T lymphocytes. Immunol. Rev. 2002; 186:172-188.
• Pribila, J.T. and Shimizu, Y.: Signal transduction events regulating integrin function and T cell migration: new functions and complexity.  Immunol. Res. 2003; 27:107-128.
• Pribila, J.T., Quale, A.C., Mueller, K.L. and Shimizu, Y.: Integrins and T cell-mediated immunity. Annu. Rev. Immunol. 2004; 22:157-180.
• Pribila, J.T., Itano, A.A., Mueller, K.L. and Shimizu, Y.: The alpha1beta1 and alphaEbeta7 integrins define a subset of dendritic cells in peripheral lymph nodes with unique adhesive and antigen uptake properties. J. Immunol. 2004;172:282-291.
• Goda, S., Quale, A.C., Woods, M.L., Felthauser, A. and Shimizu, Y.: Control of TCR-mediated activation of beta1 integrins by the ZAP-70 interdomain B region and the linker for activation of T cells adapter protein. J. Immunol. 2004; 172:5379-5387.
• Mueller, K.L., Daniels, M.A., Felthauser, A., Kao, C., Jameson, S.C. and Shimizu, Y.: Cutting edge: LFA-1 integrin-dependent T cell adhesion is regulated by both Ag specificity and sensitivity. J. Immunol. 2004; 173:2222-2226.
• Rosenthal-Allieri, M.A., Ticchioni, M., Breittmayer, J.P., Shimizu, Y. and Bernard, A.: Influence of beta1 integrin intracytoplasmic domains in the regulation of VLA-4-mediated adhesion of human T cells to VCAM-1 under flow conditions. J. Immunol. 2005; 175:1214-1223.
• Medeiros, R.B., Dickey, D.M., Chung, H., Quale, A.C., Nagarajan, L.R., Billadeau, D.B. and Shimizu, Y.: Protein kinase D1 and the beta1 integrin cytoplasmic domain control beta1 integrin function via regulation of Rap1 activation. Immunity 2005; 23:213-226.
• Finkelstein, L.D., Shimizu, Y. and Schwartzberg, P.L. Tec kinases regulate T cell receptor-mediated recruitment of signaling molecules and integrin-dependent cell adhesion. J. Immunol. 2005; 175:5923-5930.