Timothy P. Singleton, M.D.

Associate Professor
612-273-5464
sing0030@umn.edu

Educational Background

• University of Minnesota, Duluth, Minnesota (1982), B.S. (Biology and Chemistry)
• University of Minnesota Medical School, Minnesota (1987), M.D.
• University of Minnesota Medical Center, Minnesota (1991), Resident (Anatomic and Clinical Pathology)
• Stanford University Medical Center, California (1992), Fellow (Surgical Pathology)
• University of Minnesota Medical Center, Minnesota (1993), Fellow (Hematopathology)

Board Certification

• Diplomate, American Board of Pathology in Anatomic and Clinical Pathology, 1993 – Present
• Diplomate, American Board of Pathology in Hematology, 1997 – Present

Professional Background

• Clinical Instructor and Staff Physician, Stanford University Medical Center, Department of Pathology, California, 1992-1994
• Assistant Director and Co-Director, University of Michigan Hospitals, Clinical Immunohistochemistry, Ann Arbor, Michigan, 1994-2000
• Assistant Professor, University of Michigan Medical School, Pathology (Hematopathology), Ann Arbor, Michigan, 1994-2000
• Chief, William Beaumont Hospital, Flow Cytometry, Royal Oak, Michigan, 2000-2004
• Director, University of Minnesota Medical Center, Fairview, Hematopathology – Flow Cytometry, Minneapolis, MN, 2004 - Present

Professional Memberships

• American Medical Association
• American Society of Hematology
• Clinical Cytometry Society
• Society for Hematopathology
• United States and Canadian Academy of Pathology

Publications

• Elimelakh M, Dayton V, Park KS, Gruessner RW, Gruessner AC, Sutherland D, Bland B, Howe RB, van Burik J, Ashrani AA, Reding MT, Singleton TP, Key NS Acquired Pure Red Cell Aplasia Associated with Alemtuzumab, Mycophenolate, and Daclizumab Immunosuppression after Pancreas Transplant (accepted for 2005 American Society of Hematology)
• Cioc AM, Dolan MM, and Singleton TP. Myeloid/natural killer cell precursor immunophenotype is present in a heterogeneous group of acute leukemias (accepted for 2006 United States and Canadian Academy of Pathology)
• Alvarez H, Dayton VD, Dolan MM, and Singleton TP. Atypical Blast Morphology at Relapse of Acute Promyelocytic Leukemia (accepted for 2006 United States and Canadian Academy of Pathology)
• Cioc AM, Singleton TP, and Dolan MM. Aplastic Anemia May Acquire Monosomy 7 Associated with Dysthrombopoiesis (accepted for 2006 United States and Canadian Academy of Pathology)
• Smith MD, Singleton TP, Balaraman S, Jaiyesimi I, O'Malley B, Al-Saadi A, and Mattson JC. Case report: mantle cell lymphoma, prolymphocytoid variant, with leukostasis syndrome. Modern Pathology 17(7):879-83, 2004.
• Chen JC, Zhang X, Singleton TP. Kiechle FL. Mitochondrial membrane potential change induced by Hoechst 33342 in myelogenous leukemia cell line HL-60. Annals of Clinical & Laboratory Science 34(4):458-66, 2004.
• Kansal R, CW Ross, TP Singleton, WG Finn and B Schnitzer. Histopathologic features of splenic small B-cell lymphomas. A study of 42 cases with a definitive diagnosis by the World Health Organization classification. Am J Clin Pathol 120:335-47, 2003.
• Valdez R, SH Kroft, CW Ross, B Schnitzer, TP Singleton, LC Peterson and WG Finn. Cerebrospinal fluid involvement in mantle cell lymphoma. Mod Pathol 15:1073-9, 2002.
• Trask DK, GT Wolf, CR Bradford, SG Fisher, K Devaney, M Johnson, T Singleton and M Wicha. Expression of Bcl-2 family proteins in advanced laryngeal squamous cell carcinoma: correlation with response to chemotherapy and organ preservation. Laryngoscope 112:638-44, 2002.
• Uherova P, CW Ross, WG Finn, TP Singleton, R Kansal and B Schnitzer. Peripheral T-cell lymphoma mimicking marginal zone B-cell lymphoma. Mod Pathol 15:420-5, 2002.
• Hans CP, WG Finn, TP Singleton, B Schnitzer and CW Ross. Usefulness of anti-CD117 in the flow cytometric analysis of acute leukemia. Am J Clin Pathol 117:301-5, 2002.
• Kansal R, TP Singleton, CW Ross, WG Finn, RF Padmore and B Schnitzer. Follicular hodgkin lymphoma: a histopathologic study. Am J Clin Pathol 117:29-35, 2002.
• Valdez R, WG Finn, CW Ross, TP Singleton, JA Tworek and B Schnitzer. Waldenstrom macroglobulinemia caused by extranodal marginal zone B-cell lymphoma: a report of six cases. Am J Clin Pathol 116:683-90, 2001.
• Ofotokun I, C Carlson, SD Gitlin, G Elta, TP Singleton and DM Markovitz. Acute cytomegalovirus infection complicated by vascular thrombosis: a case report. Clin Infect Dis 32:983-6, 2001.
• Uherova P, CW Ross, B Schnitzer, TP Singleton and WG Finn. The clinical significance of CD10 antigen expression in diffuse large B-cell lymphoma. Am J Clin Pathol 115:582-8, 2001.
• Finn WG, TP Singleton, B Schnitzer, CW Ross and LM Stoolman. Adhesion molecule expression in CD5-negative/CD10-negative chronic B-cell leukemias: comparison with non-Hodgkin's lymphomas and CD5-positive B-cell chronic lymphocytic leukemia. Hum Pathol 32:66-73, 2001.
• Izban KF, S Alkan, TP Singleton and ED Hsi. Multiparameter immunohistochemical analysis of the cell cycle proteins cyclin D1, Ki-67, p21WAF1, p27KIP1, and p53 in mantle cell lymphoma. Archiv Pathol Lab Med 124:1457-62, 2000.
• Bavikatty NR, CW Ross, WG Finn, B Schnitzer and TP Singleton. Anti-CD10 immunoperoxidase staining of paraffin-embedded acute leukemias: comparison with flow cytometric immunophenotyping. Hum Pathol 31:1051-4, 2000.
• Kroft SH, R Domiati-Saad, WG Finn, DB Dawson, B Schnitzer, TP Singleton and CW Ross. Precursor B-lymphoblastic transformation of grade I follicle center lymphoma. Am J Clin Pathol 113:411-8, 2000.
• Hsi ED, TP Singleton, L Swinnen, CH Dunphy and S Alkan. Mucosa-associated lymphoid tissue-type lymphomas occurring in post-transplantation patients. Am J Surg Pathol 24:100-6, 2000.
• Iravani S, TP Singleton, CW Ross and B Schnitzer. Precursor B lymphoblastic lymphoma presenting as lytic bone lesions. Am J Clin Pathol 112:836-43, 1999.
• Kleer CG, KJ Wojno, K Fields and TP Singleton. Detection of Estrogen Receptor in Carcinomas of the Breast Using Automated Immunohistochemistry. Appl Immunohistochem and Molecul Morphol 7:103-107, 1999.
• Singleton TP, MM Anderson, CW Ross and B Schnitzer. Leukemic phase of mantle cell lymphoma, blastoid variant. Am J Clin Pathol 111:495-500, 1999.
• Kroft SH, ED Hsi, CW Ross, B Schnitzer and TP Singleton. Evaluation of CD23 expression in paraffin-embedded gastric lymphomas of mucosa-associated lymphoid tissue. Mod Pathol 11:967-70, 1998.
• Tworek JA, TP Singleton, B Schnitzer, ED Hsi and CW Ross. Flow cytometric and immunohistochemical analysis of small lymphocytic lymphoma, mantle cell lymphoma, and plasmacytoid small lymphocytic lymphoma. Am J Clin Pathol 110:582-9, 1998.
• Hsi ED, TP Singleton, SM Svoboda, B Schnitzer and CW Ross. Characterization of the lymphoid infiltrate in Hashimoto thyroiditis by immunohistochemistry and polymerase chain reaction for immunoglobulin heavy chain gene rearrangement. Am J Clin Pathol 110:327-33, 1998.
• Kroft SH, WG Finn, TP Singleton, CW Ross, S Sheldon and B Schnitzer. Follicular large cell lymphoma with immunoblastic features in a child with Wiskott-Aldrich syndrome: an unusual immunodeficiency-related neoplasm not associated with Epstein-Barr virus. Am J Clin Pathol 110:95-9, 1998.
• Hsi ED, A Eisbruch, JK Greenson, TP Singleton, CW Ross and B Schnitzer. Classification of primary gastric lymphomas according to histologic features. Am J Surg Pathol 22:17-27, 1998.
• Su LD, B Schnitzer, CW Ross, M Vasef, S Mori, M Shiota, DY Mason, K Pulford, JT Headington and TP Singleton. The t(2;5)-associated p80 NPM/ALK fusion protein in nodal and cutaneous CD30+ lymphoproliferative disorders. J Cutan Pathol 24:597-603, 1997.
• Kroft SH, TP Singleton, M Dahiya, CW Ross, B Schnitzer and ED Hsi. Ruptured spleens with expanded marginal zones do not reveal occult B-cell clones. Mod Pathol 10:1214-20, 1997.
• Khalidi HS, TP Singleton and SW Weiss. Inflammatory malignant fibrous histiocytoma: distinction from Hodgkin's disease and non-Hodgkin's lymphoma by a panel of leukocyte markers. Mod Pathol 10:438-42, 1997.
• Tworek JA, HD Appelman, TP Singleton and JK Greenson. Stromal tumors of the jejunum and ileum. Mod Pathol 10:200-9, 1997.
• Svoboda-Newman SM, JK Greenson, TP Singleton, R Sun and TS Frank. Detection of hepatitis C by RT-PCR in formalin-fixed paraffin- embedded tissue from liver transplant patients. Diagn Mol Pathol 6:123-9, 1997.
• Hsi ED, JK Greenson, TP Singleton, J Siddiqui, B Schnitzer and CW Ross. Detection of immunoglobulin heavy chain gene rearrangement by polymerase chain reaction in chronic active gastritis associated with Helicobacter pylori. Hum Pathol 27:290-6, 1996.
• Anderson MM, CW Ross, TP Singleton, S Sheldon and B Schnitzer. Ki-1 anaplastic large cell lymphoma with a prominent leukemic phase. Hum Pathol 27:1093-5, 1996.
• Cooney KA, JC Wetzel, SD Merajver, JA Macoska, TP Singleton and KJ Wojno. Distinct regions of allelic loss on 13q in prostate cancer. Cancer Res 56:1142-5, 1996.
• Singleton TP, T Perrone, G Oakley, GA Niehans, L Carson, SS Cha and JG Strickler. Activation of c-erbB-2 and prognosis in ovarian carcinoma. Comparison with histologic type, grade, and stage. Cancer 73:1460-6, 1994.
• Nguyen PL, PE Swanson, W Jaszcz, DM Aeppli, G Zhang, TP Singleton, S Ward, D Dykoski, J Harvey and GA Niehans. Expression of epidermal growth factor receptor in invasive transitional cell carcinoma of the urinary bladder. A multivariate survival analysis. Am J Clin Pathol 101:166-76, 1994.
• Niehans GA, TP Singleton, D Dykoski and DT Kiang. Stability of HER-2/neu expression over time and at multiple metastatic sites. J Natl Cancer Inst 85:1230-5, 1993.
• Mahoney CA, N Sherwood, EH Yap, TP Singleton, DJ Whitney and PJ Cornbleet. Ciliated cell remnants in peritoneal dialysis fluid. Arch Pathol Lab Med 117:211-3, 1993.
• Goswitz JJ, KE Willard, SJ Eastep, CJ Shanholtzer, ML Olson, M Pinnell, T Singleton and LR Peterson. Utility of slide centrifuge gram's stain versus quantitative culture for diagnosis of urinary tract infection. Am J Clin Pathol 99:132-6, 1993.
• Crary GS, TP Singleton, JP Neglia, PE Swanson and JG Strickler. Detection of metastatic neuroblastoma in bone marrow biopsy specimens with an antibody to neuron-specific enolase. Mod Pathol 5:308-11, 1992.
• Singleton TP, GA Niehans, F Gu, CE Litz, K Hagen, Q Qiu, DT Kiang and JG Strickler. Detection of c-erbB-2 activation in paraffin-embedded tissue by immunohistochemistry. Hum Pathol 23:1141-50, 1992.
• Singleton TP and JG Strickler. Clinical and pathologic significance of the c-erbB-2 (HER-2/neu) oncogene. Pathol Annu 1:165-190, 1992.
• George E, GA Niehans, PE Swanson, JG Strickler and TP Singleton. Overexpression of the c-erbB-2 oncogene in sarcomas and small round-cell tumors of childhood. An immunohistochemical investigation. Arch Pathol Lab Med 116:1033-5, 1992.
• Strickler JG, TP Singleton, CM Copenhaver, A Erice and DC Snover. Adenovirus in the gastrointestinal tracts of immunosuppressed patients. Am J Clin Pathol 97:555-8, 1992.
• Smith MD and Singleton TP. Circulating B-cells that lack surface immunoglobulin light chain usually represent lymphoma/leukemia. Modern Pathology 17(supplement 1): 271A, 2004.
• Parling-Lynch KJ, Zakalik D, Hwang R, Singleton TP, and Wong CYO. Significant differences of standard uptake values (SUV) from PET scan between indolent and aggressive lymphomas. Blood 102(11, part 2):270b, 2003.
• Smith MD, Mattson JC and Singleton TP. Acute bilineal leukemia, B-precursor lymphoblastic and monocytic, with del (11)(q23). (Society for Hematopathology and the European Association for Hematopathology 2003 Workshop, Memphis, TN.)
• Smith MD, Mattson JC and Singleton TP. Acute promyelocytic leukemia. (Society for Hematopathology and the European Association for Hematopathology 2003 Workshop, Memphis, TN.)
• Chen JC and Singleton T. Quantitation of Platelet Associated Immunoglobulin by Antibody Binding Capacity. (Great Lakes International Imaging and Flow Cytometry Association Meeting, Milwaukee, Wisconsin, 2003.)
• Selby D, Padmore R, Tuck M, Ross C, Schnitzer B, Finn W, Valdez R, Singleton T, and Kaminski M.  Characterization of Bone Marrow Lymphoid Aggregates in Follicular Lymphoma Treated with [131I] Anti-B1 Antibody. Mod Pathol 16(1):251A, 2003.
• Pandya SN and Singleton TP. Hepatosplenic gamma delta T-cell lymphoma. (Check Sample for American Society of Clinical Pathologists, 2003)
• Chen JC, Singleton T, Fosdick T, Eisenbrey AB.  Comparison of a mixture versus a single anti-HLA-B27 antibody reagent in flow cytometric HLA-B27 typing. Cytometry 50(5):280-281, 2002.
• Singleton TP and Phadke DM.  Flow cytometric patterns in myelodysplastic syndromes (MDS). Mod Pathol 15(1):264A, 2002.
• Chen JC, Davis BH, Bigelow N, and Singleton T, A No-Wash Flow Cytometric Assay for Fetal Red Blood Cells. Cytometry 46:314, 2001.
• Chen JC, Zhang X, Singleton T, and Kiechle FL. Hoechst 33342 Depolarizes the Mitochondrial Transmembrane Potential in Myelogenous Leukemic Cells. Cytometry 46:314, 2001.
• Nangia R, Singleton TP, Finn WG, Schnitzer B and Ross CW.  CD117 (c-kit) in acute leukemias, chronic myeloproliferative disorders and myelodysplastic syndromes: a paraffin immunohistochemical (IHC) study with flow cytometric (FC) correlation. Mod Pathol 14(1):173A, 2001.
• Petricek CM, Ross CW, Finn WG, Schnitzer B and Singleton TP.  Utility of anti-CD117 flow cytometric analysis of acute leukemia. Mod Pathol 13(1):160A, 2000. 
• Nangia R, Singleton TP, Ross CW, Finn WG, Padmore RF and Schnitzer B.  Follicular Hodgkin’s lymphoma (FHL): A clinicopathologic study. Mod Pathol 13(1):158A, 2000.
• Uherova P, Singleton TP, Ross CW, Schnitzer B and Finn WG.  The clinical significance of CD10 antigen expression in diffuse large B-cell lymphoma. Mod Pathol 13(1):165A, 2000. 
• Finn WG, Singleton TP, Schnitzer B, Ross CW and Stoolman LM.  Adhesion molecule expression in CD5 negative (-), CD10 negative chronic B-cell leukemias.  Mod Pathol 13(1):148A, 2000. 
• Nangia R, Kroft SH, Singleton TP, Ross CW, Finn WG and Schnitzer B.  Splenic morphology in small B-cell lymphoproliferative disorders (SBL): A study of 48 well-characterized cases. Mod Pathol 13(1):158A, 2000. 
• Valdez R, Finn WG, Ross CW, Singleton TP, Tworek, JA and Schnitzer B.  Extranodal marginal zone B-cell lymphoma associated with Waldenstrom’s macroglobulinemia. Mod Pathol 13(1):165A, 2000.
• Bavikatty NR, Ross CW, Finn WG, Schnitzer B and Singleton TP.  CD10 detection in acute leukemia by immunohistochemistry in paraffin sections. Am J Clin Pathol 112(4):560, 1999.
• Bavikatty NR, Finn WG, Schnitzer B, Ross CW and Singleton TP.  Expression of CD99 and terminal deoxynucleotidyl transferase in acute myeloid leukemia. Am J Clin Pathol 112(4):561, 1999.
• Padmore RF, Finn WG, Singleton TP, Ross CW and Schnitzer B.  Fascin expression in immunophenotypic variants of classical Hodgkin’s disease. Mod Pathol 12(1):144A, 1999.
• Valdez R, Kroft SH, Schnitzer B, Ross CW, Singleton TP, Peterson LC and Finn WG.  Cerebrospinal fluid (CSF) involvement by mantle cell lymphoma. Mod Pathol 12(1):147A, 1999.
• Kroft SH, Hsi, ED, Ross CW, Schnitzer B and Singleton TP.  CD23 expression in paraffin-embedded gastric MALT lymphoma. Am J Clin Pathol 109(4):477, 1998.
• Iravani S, Ross CW, Schnitzer B and Singleton TP.  Ocular adnexal lymphomas (OAL). Lack of CD23 in nonfollicular small B-cell lymphoma supports marginal zone type. Mod Pathol 11(1):132A, 1998.
• Iravani S, Singleton TP, Ross CW and Schnitzer B.  B-cell lymphoblastic lymphoma presenting as lytic bone lesions: a clinicopathological study. Mod Pathol 11(1): 132A, 1998.
• Hsi ED, Singleton TP, Swinnen L, Greenson JK and Alkan S.  Mucosa-associated lymphoid tissue (MALT) type lymphomas occurring in posttransplantation patients: A form of posttransplantation lymphoproliferative disorder (PTLD)? Mod Pathol 11(1):131A, 1998.
• Kleer CG, Singleton TP and Wojno KJ.  Estrogen receptor expression in paraffin-embedded carcinomas of the breast: Comparison of two monoclonal antibodies using an automated stainer. Am J Clin Pathol 109(4):488, 1998.
• Pearsall CP, Kroft S, Ross C, Schnitzer B and Singleton TP.  Apoptosis in myelodysplastic syndromes. Mod Pathol 10(1):132A, 1997.
• Hsi ED, Singleton TP, Svoboda S, Frank TS, Schnitzer B and Ross CW.  Characterization of the lymphoid infiltrate in Hashimoto’s thyroiditis by immunohistochemistry (IHC) and polymerase chain reaction for immunoglobulin heavy chain gene rearrangement (IgH PCR). Mod Pathol 10(1):127A, 1997.
• Su L, Singleton TP, Schnitzer B, Mori S, Shiota M, Headington JT.  The t(2;5) translocation-associated p80 NPM/ALK fusion protein is expressed in some nodal Ki-1 anaplastic large cell lymphomas, but not in primary cutaneous Ki-1 lymphomas or lymphomatoid papulosis. Mod Pathol 10(1):47A, 1997.
• Kroft SH, Singleton TP, Dahiya M, Ross CW, Schnitzer B and Hsi ED.  Absence of occult B-cell clones within traumatically ruptured spleens with expanded marginal zones. Mod Pathol 10(1):128A, 1997.
• Book Review:  Surgical Pathology of the Lymph Nodes and Related Organs by ES Jaffe, Second Edition.  in Am J Surg Pathol  20(6):775, 1996.
• Poston CD, Ross CW, Schnitzer B and Singleton TP.  Phenotypic analysis of acute leukemias by immunohistochemistry on bone marrow, flow cytometry (FC) and morphology. Mod Pathol 9(1):120A, 1996.
• Tworek JA, Singleton TP, Schnitzer B, Hsi ED and Ross CW.  Immunophenotypic analysis of small lymphocytic lymphoma (SLL), plasmacytoid SLL (SLLP), and mantle cell lymphoma (MCL). Mod Pathol 9(1):124A, 1996.
• Khalidi HS, Singleton TP and Weiss SW.  Do Leukocyte Markers Distinguish Inflammatory Malignant Fibrous Histiocytoma from Hodgkin's and non-Hodgkin's Lymphoma? Mod Pathol 9(1):8A, 1996.
• Singleton TP, Frank TS, Fields K, Sun R and Wojno KJ. Immunohistochemistry (IHC) for estrogen receptor (ER) using an automated stainer:  comparison with IHC by hand and with the cytosol assay. Mod Pathol 9(1):175A, 1996.
• Hsi ED, Greenson JK, Singleton TP, Eisbruch A, Ross CW and Schnitzer B.  Classification of primary gastric lymphomas according to histologic features. Mod Pathol 9(1):59A, 1996.
• Svoboda SM, Greenson JK, Singleton TP, Sun R and Frank TS. Detection of hepatitis C in paraffin sections of formalin fixed liver using RT-PCR. Mod Pathol 9(1):137A, 1996.
• Tworek JA, Appelman HD, Singleton TP and Greenson JK.  Stromal tumors of the jejunum and ileum. Mod Pathol 9(1):67A, 1996.
• Lee CT, Wojno K, Oesterling JE, Singleton T, McCauley L, Lehr J, Montie JE, and Pienta K. Expression of parathrydoid hormone-like protein in prostate cancer and prostatic intraepithelial neoplasia. Proc Annu Meet Am Assoc Cancer Res 37:A1676, 1996. (Resident Award Poster)
• Cooney KA, Wetzel J, Merjaver SD, Macoska JA, Singleton TP, Wojno KJ.  Loss of Heterozygosity Involving Chromosome 13q14.3 in Sporadic Prostate Cancer.  (American Cancer Society, April 1995.)
• Blaivas M, Mikhail A, Perry A, McKeever PE, Singleton T, Scheithauer BW.  Macrophages in high grade gliomas with or without associated infarct.  (American Association of Neuropathologists, 1996)
• Anderson MM, Singleton TP, Ross CW, Sheldon S, Schnitzer B. Leukemic phase of mantle cell lymphoma, blastic variant. Mod Pathol 8(1):105A, 1995.
• Hsi ED, Greenson JK, Singleton TP, Siddiqui J, Schnitzer B, Ross CW.  Detection of immunoglobulin heavy chain (IgH) gene rearrangement by polymerase chain reaction (PCR) in chronic active gastritis associated with Helicobacter pylori (CAG-Hp). Mod Pathol 8(1):113A, 1995.  (Winner of the Stowell-Orbison Award for pathologists-in-training) 
• Anderson MM, Sheldon S, Singleton TP, Ross CW Schnitzer B.  Demonstration of t(2;5) is important in the diagnosis of Ki-1 anaplastic large cell lymphoma (ALCL) with unusual features. Mod Pathol 8(1):105A, 1995.
• Stevens SR, Cooper KD, Wahl RL, Ross CW, Singleton TP, Kaminski MS. Radioimmunotherapy of cutaneous B-cell lymphoma with [131I] anti-B1 (anti-CD20) antibody induces a novel cutaneous reaction pattern associated with complete remission of cutaneous disease. (Society for Investigative Dermatology, 1995)
• Cohen, Singleton TP.  Increased TGF-beta immunolocalization in unhealed burn wounds. (Plastic Surgery, 1995)
• George E, Niehans GA, Swanson PE, Strickler JG and Singleton TP.  Overexpression of the c-erbB-2 oncogene in sarcomas and small round cell tumors of childhood.  An immunohistochemical investigation.  (United States and Canadian Academy of Pathology, 1991)