Mark Osborn, Ph.D.

Assistant Professor
Department of Pediatrics
Division of Blood and Marrow Transplantation
Mayo Mail Code 366
420 Delaware St. SE
Minneapolis, MN 55455
Phone (612) 625-8327
Fax (612) 626-2815
osbor026@umn.edu
Preferred method of contact: email

Dr. Osborn is an Assistant Professor in the Department of Pediatrics, Division of Blood and Marrow Transplantation. He is also a member of the Cancer Center.

Dr. Osborn received his PhD degree from the University of Minnesota in 2009 in the laboratory of Dr. Bruce R. Blazar, MD.

Research Interests

Gene Therapy
- Non-viral gene transfer for the treatment of inborn errors of metabolism.

Genome Editing
- Correction of disease specific mutations in a precise manner using homologous recombination.

Cellular Therapy
- Ex vivo correction of murine and human adult stem cells.

Honors and Awards

• 2005-2007 American Heart Association Pre-doctoral Fellowship

 Selected recent publications

Osborn M.J., Ryan P.L., Kirchhof N., Panoskaltsis-Mortari A., Mortari F., Tudor K.S. Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis. Blood. 2004 Feb 1;103(3):843-51.

Tolar J., Osborn M.J., Bell S., McElmurry R., Xia L., Riddle M., Panoskaltsis-Mortari A., Jiang Y., McIvor R.S., Contag C.H., Yant S.R., Kay M.A., Verfaillie C.M., Blazar B.R. Real-time in vivo imaging of stem cells following transgenesis by transposition. Mol Ther. 2005 Jul;12(1):42-8.

Osborn M.J., Panoskaltsis-Mortari A., McElmurry R.T., Bell S.K., Vignali D.A., Ryan M.D., Wilber A.C., McIvor R.S., Tolar J., Blazar B.R. A picornaviral 2A-like sequence-based tricistronic vector allowing for high-level therapeutic gene expression coupled to a dual-reporter system. Mol Ther. 2005 Sep;12(3):569-74.

Tolar J., Nauta A.J., Osborn M.J., Panoskaltsis Mortari A., McElmurry R.T., Bell S., Xia L., Zhou N., Riddle M., Schroeder T.M., Westendorf J.J., McIvor R.S., Hogendoorn P.C., Szuhai K., Oseth L., Hirsch B., Yant S.R., Kay M.A., Peister A., Prockop D.J., Fibbe W.E., Blazar B.R. Sarcoma derived from cultured mesenchymal stem cells. Stem Cells. 2007 Feb;25(2):371-9

Osborn, M.J., McElmurry, R.T., Peacock, B., Tolar, J., and Blazar, B.R. Targeting of the CNS in MPS-IH using a nonviral transferrin-alpha-L-iduronidase fusion gene product. Mol Ther. 2008 Aug;16(8):1459-66. PMCID: 2574880

Tolar J., Ishida-Yamamoto A., Riddle M., McElmurry R.T., Osborn M.J., Xia L., Lund T., Slattery C., Uitto J., Christiano A.M., Wagner J.E., Blazar B.R. Amelioration of epidermolysis bullosa by transfer of wild-type bone marrow cells. Blood. 2009 Jan 29;113(5):1167-74. PMCID: 2635082

Kelly RM, Goren EM, Taylor PA, Mueller SN, Stefanski HE, Osborn M.J., Scott, HS, Komarova EA, Gudkov AV, Hollander GA, and Blazar BR. 2009. “Short-Term Inhibition of p53 Combined with Keratinocyte Growth Factor Improves Thymic Epithelial Recovery and Enhances T-cell Reconstitution After Murine Bone Marrow Transplantation”, Blood. 2010 Feb 4; 11 (5): 1088-97.

Wagner JE, Ishida-Yamamoto I., McGrath JA, Hordinsky M., Keene DR, Riddle M., Osborn MJ, Lund TC, Dolan M., Blazar BR, Tolar J. Allogeneic Bone Marrow Transplantation Leads to Type VII Collagen Deposition and Clinical Improvement in Patients with Recessive Dystrophic Epidermolysis Bullosa. New England Journal of Medicine. 2010 Aug; 363: 629-639.

Tolar J, Park IH, Xia L, Lees CJ, Peacock B, Webber B, McElmurry RT, Eide CR, Orchard PJ, Kyba M, Osborn MJ, Lund TC, Wagner JE, Daley GQ, Blazar BR. Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome). Blood. 2010 Oct 29

Osborn MJ, McElmurry RT, Lees CJ, Defeo AP, Chen ZY, Kay MA, Naldini L, Freeman G, Tolar J, Blazar BR.Minicircle DNA-based Gene Therapy Coupled With Immune Modulation Permits Long-term Expression of α-L-Iduronidase in Mice With Mucopolysaccharidosis Type I. Mol. Ther. 2010 Nov 16.

Tolar J, Xia L, Riddle MJ, Lees CJ, Eide CR, McElmurry RT, Titeux M, Osborn MJ, Lund TC, Hovnanian A, Wagner JE, Blazar BR. Induced Pluripotent Stem Cells from Individuals with Recessive Dystrophic Epidermolysis Bullosa. J. Invest. Dermatol. 2010 DEC 2.

Osborn MJ, Defeo AP, Blazr, BR, Tolar, J Synthetic Zinc Finger Nuclease Design and Rapid Assembly. Hum. Gene Ther. 2011 25 JUL.