Cheryl A. Gale, M.D.

MMC 39, D-136 Mayo
420 Delaware St. SE
Minneapolis, MN 55455
612-624-1982 Tel
612-624-8176 Fax
galex012@umn.edu

Cheryl A. Gale, M.D. is an assistant professor in the Division of Neonatology. She earned her M.D. from the University of Minnesota, and served her internship and residency at Washington University-St. Louis Children’s Hospital in St. Louis before returning to the University of Minnesota to complete her Neonatal-Perinatal Medicine Fellowship. In addition to attending on the Neonatal Intensive Care Unit at Fairview-University Medical Center, Dr. Gale conducts research in the areas of Candida albicans morphogenesis and pathogenesis and has two US patents from her research work. She has published in Proceedings of the National Academy of Sciences USA and in Science and Molecular Biology of the Cell, among others. Dr. Gale held the Minnesota Chapter Research Award from the March of Dimes from 2000 to 2002.

CURRICULUM VITAE FOR CHERYL GALE, M.D. (last revised 11/17/04)

EDUCATION
1985 B.S. Summa cum laude, Biochemistry, University of Minnesota; Minneapolis, Minnesota
1989 M.D. University of Minnesota Medical School; Minneapolis, Minnesota

POSTDOCTORAL TRAINING
1989-92 Pediatric Internship and Residency Program; Washington University – St. Louis Children’s Hospital; St. Louis, Missouri
1992-96 Neonatal-Perinatal Medicine Fellowship Program; University of Minnesota; Minneapolis, Minnesota

BOARD CERTIFICATION (RECERTIFICATION)
American Board of Pediatrics – Pediatrics 1995-2002, 2003-2010
American Board of Pediatrics – Neonatal-Perinatal Medicine 1997-2004, 2005-2012

ACADEMIC APPOINTMENTS
1996-99 Instructor, Department of Pediatrics; University of Minnesota
1999 Assistant Professor, Department of Pediatrics; University of Minnesota
1999 Associate member, Graduate School Faculty, Biological Sciences; University of Minnesota
2003 Assistant Professor, Department of Genetics, Cell Biology, and Development; University of Minnesota

AWARDS AND HONORS
Undergraduate and Graduate
1984-85 Biological Sciences Alumni Society Scholarship
1984-85 Francis Shepherd Memorial Scholarship for Outstanding Women Students
1984-85 Henrietta Novy-Miller Scholarship for Outstanding Biochemistry Student
1985 University of Minnesota Research Opportunities Program Grant, Department of Biochemistry, Laboratory of Irvin E. Liener, Ph.D.
1988 Minnesota Medical Foundation Research Grant, University of Minnesota, Department of Pediatrics, Laboratory of Margaret K. Hostetter, M.D.

Postdoctoral
1993-96 St. Jude Children’s Hospital and NIH Research Fellowship through the Pediatric Scientist Development Program, Department of Pediatrics, University of Minnesota, Laboratory of Margaret K. Hostetter, M.D.
1995 First Place, University of Minnesota, Department of Pediatrics Fellows’ Research Symposium for presentation of “A Candida albicans gene with integrin motifs induces hyphal-like structures in S. cerevisiae”
1996 First Place, University of Minnesota, Department of Pediatrics Fellows’ Research Symposium for presentation of “Monoallelic disruption of INT1 reduces antibody binding, adhesion and germ tube formation in C. albicans”
1996 Winner of the Society for Pediatric Research Fellow’s Basic Science Research Award for the abstract “Monoallelic disruption of INT1 reduces antibody binding, adhesion, and germ tube formation in C. albicans,” Gale CA, Olson J, Hostetter MK
1996-99 National Institutes of Health Child Health Research Center Scholar, Department of Pediatrics, University of Minnesota, Laboratory of Judith Berman, Ph.D.
1997-98 University Children’s Foundation Scholar
2000-02 Basil O’Connor Scholar, March of Dimes
2001-present Member, Society for Pediatric Research
2002 Alexander Charles Jundt Research Award
2002-04 National Institutes of Health Pediatric Scientist LRP Award (competitive application)
2004 Fellow, American Academy of Pediatrics
2004-05 National Institutes of Health Pediatric Scientist LRP Award (competitive application)

GRANT SUPPORT
Current
2002-07 National Institutes of Health RO1 DE014666. Role: Co-investigator. “Morphogenesis and Cell Cycle Regulation in Candida albicans.” (Judith Berman, P.I) 2% salary support/year.

2003-05 University of MN Viking Children’s Fund Award, $50,000, “The Role of the Bud1p-GTPase Module in Candida albicans Morphogenesis.”  Role: Principal Investigator.

Past
1997-99 University Children’s Foundation Award, University of Minnesota, $50,000, “Molecular models of organogenesis.”  Role: Principal Investigator.

1998 Viking Children’s Fund Award, University of Minnesota, $8,500, “A molecular mechanism for morphologic differentiation in Candida albicans.”  Role: Principal Investigator.

1999-2000 University of Minnesota Graduate School Grant-in-Aid of Research and Artistry, $25,000, “The role of SWE1 in morphologic switching in Candida albicans.” Role: Principal Investigator.

1999-2000 Minnesota Medical Foundation, $15,000, “The role of SWE1 in morphologic switching in Candida albicans.” Role: Principal Investigator.

2000-02 March of Dimes Basil O’Connor Starter Scholar Award, $100,000, “In vivo localization of morphogenetic proteins in Candida albicans.” Role: Principal Investigator.

2001-02 Viking Children's Fund, $10,000. 

2001-02 Minnesota Medical Foundation, role: Principal Investigator, “The Role of Morphogenesis Checkpoints in Candida albicans Morphogenesis and Virulence,” $15,000. Role: Principal Investigator.

2000-03 National Institutes of Health Mentored Clinical Scientist Award (K08). Role: Principal Investigator. “A Molecular Model of Morphologic Switching in Candida albicans.” Total direct costs: $351,000.

2002-03 University of Minnesota Graduate School Grant-in-aid of Research and Artistry, $19,887, “Characterization of the cytoskeletal targets of morphogenesis signaling pathways in Candida albicans.”  Role: Principal Investigator.

PATENTS
Candida Albicans Integrin-like protein; US patent # 5,886,151
DNA encoding Candida albicans Integrin-like protein; US patent # 6,346,411
Candida albicans gene, Integrin-like protein, antibodies, and methods of use; US patent # 6,774,219

ACADEMIC ACTIVITIES/TEACHING
1999 Research advisor for Bhuma Krishnamachari (Masters in Biological Sciences)
2000 Reviewer for Microbiology, U.K.
2001 Instructor of “Fungal Pathogenesis” within the course “Microbial Pathogenesis” (Microbiology 4151)
2001 Research and Academic Mentor for Katherine Swanson (high school senior participating in the Mentor Connection Program through Independent School District 279)
2002-present Co-chair/organizer U of MN Dept. of Pediatrics Fellows’ Research Symposium
2002 Instructor at the Molecular Mycology course at Woods Hole, MA.
2003 Reviewer for Clinical Infectious Diseases
2003 Research mentor for: Michelle Waste (summer undergraduate research), Nicholas Winning (undergraduate directed research), and Elizabeth Masko (undergraduate honors thesis research)
2004 Ad hoc grant reviewer for The Department of Veterans Affairs, Washington, DC
2004 Reviewer for Molecular and Cellular Biology
2004 Research mentor for: Cassandra Kistler-Andersen (awarded a U of MN         Undergraduate Research Opportunities Program grant), Nicholas Winning and Nicole Dauer (undergraduate directed research), and Ani Rao (high school honors research)

INVITED LECTURES
September, 2001 Human Fungal Pathogens Conference in Seefeld, Austria.
August, 2002 Molecular Mycology Course in Wood’s Hole, MA.

PUBLICATIONS
1. Hostetter MK, Tao N-J, Gale CA, Herman DJ, McClellan M, Sharp RL, Kendrick KE. Antigenic and functional conservation of an integrin I-domain in Saccharomyces cerevisiae. Biochemical and Molecular Medicine 55:122-130, 1995.

2. Gale CA, Finkel D, Tao N-J, Meinke M, McClellan M, Olson J, Kendrick K, Hostetter MK. Cloning and expression of a gene encoding an integrin-like protein in Candida albicans. Proceedings of the National Academy of Sciences, USA. 95:357-361, 1996.

3. Gale CA, Bendel C, McClellan M, Hauser M, Becker J, Berman J, Hostetter M. Linkage of adhesion, filamentous growth, and virulence in Candida albicans to a single gene, INT1. Science 279:1355-1358, 1998.

4. Bendel CM, Kinneberg KM, Jechorek RP, Gale CA, Erlandsen SL, Hostetter MK, Wells CL. Systemic infection following intravenous inoculation of mice with Candida albicans int1 mutant strains. Molecular Genetics and Metabolism 67:343-351, 1999.

5. Kinneberg KM, Bendel CM, Jechorek RP, Cebelinski EA, Gale CA, Berman JG, Erlandsen SL, Hostetter MK, Wells CL. Effect of INT1 gene on Candida albicans murine intestinal colonization. The Journal of Surgical Research 87:245-251, 1999.

6. Asleson C, Bensen E, Gale CA, Melms A-S, Kurischko C, Berman J. Candida albicans INT1-induced filamentation in Saccharomyces cerevisiae depends on Sla2p. Molecular and Cellular Biology 21:1272-1284, 2001.

7. Gerami-Nejad M, Berman J, Gale CA. Cassettes for PCR-mediated construction of green, yellow, and cyan fluorescent protein fusions in Candida albicans. Yeast 18:859-864, 2001.

8. Gale CA, Gerami-Nejad M, McClellan M, Vandoninck S, Longtine MS, Berman J. Candida albicans Int1p interacts with the septin ring in yeast and hyphal cells. Molecular Biology of the Cell 12:3536-3549, 2001.

9. Gerami-Nejad M, Hausauer D, Berman J, Gale CA. Cassettes for the PCR-mediated construction of regulatable alleles in Candida albicans. Yeast 21: 429-436, 2004.

10. Prill SKH, Klinkert B, Timpel C, Gale CA, Schroppel K, Ernst J.  PMT gene family of Candida albicans: five isoforms of protein mannosyltransferase regulate growth, morphogenesis and antifungal resistance. Molecular Microbiology, in press, 2004.

11. Lee S, Khalique Z, Gale CA, and Wong B. Intracellular trafficking of fluorescently tagged proteins associated with pathogenesis in Candida albicans.  Medical Mycology, in press, 2004.

12. Crampin H, Court H, Finley K, Gerami-Nejad M, Gale CA, Berman J, and Sudbery P.  Polarized growth in Candida albicans hyphae is driven by a Spitzenkörper.  Submitted to Journal of Cell Science, November 2004.

13. Hausauer D, Gerami-Nejad M, Kistler-Anderson C, Dauer N, and Gale CA.  Bud2p, a cortical landmark protein, affects invasiveness and morphogenesis in Candida albicans.  In preparation.

14. Desrussieux M, Finley K, Gale CA, and Berman J.  Candida albicans Sla2p is involved in maintaining synchrony between the morphogenesis and nuclear cell cycles.  In preparation.

REVIEW ARTICLES
1. Davis D, Gale CA, Berman J, Magee P. Molecular biological and genomic approaches to the study of medically important fungi. Infection and Immunity 71:2299-2309, 2003.

ABSTRACTS ACCEPTED FOR PRESENTATION (first or senior author)
1. Gale CA, Hostetter MK. A Candida albicans gene with integrin motifs induces hyphal-like structures in S. cerevisiae. Pediatr Res 37:174A, 1995. Presented at the Society for Pediatric Research meeting, San Diego, CA, May 1995.

2. Gale CA, Finkel D, Tao N-J, Meinke M, McClellan M, Olson J, Hostetter M. Cloning and expression of a gene encoding an integrin-like protein in Candida albicans. Presented at the Keystone Symposium “Integrins and Signaling Events in Cell Biology and Disease,” Keystone, CO, January 1996.

3. Gale CA, Olson J, Hostetter MK. Monoallelic disruption of aINT1 reduces antibody binding, adhesion, and germ tube formation in C. albicans. Pediatr Res 39:171A, 1996. Presented at the Society for Pediatric Research meeting, Washington, D.C., May 1996. Winner of the 1996 Society for Pediatric Research Fellow’s Basic Science Research Award.

4. Gale CA, Bendel C, Hostetter M. Induction of epithelial adhesion and morphologic switching by a single gene from Candida albicans. Pediatr Res 41:119A, 1997. Presented at the Society for Pediatric Research meeting, Washington D.C., May 1997.

5. Gale CA, Bendel C, Aselson CM, Berman J, Hostetter MK. Induction of elongated branched germ tubes and adhesion to epithelial cells in S. cerevisiae by a single gene from Candida albicans. Presented at the American Society for Cell Biology meeting, Washington, D.C., December 1997.

6. Gale CA, Berman J. Characterization of Candida albicans INT1-induced filaments in Saccharomyces cerevisiae. Presented at the 98th American Society for Microbiology meeting, Atlanta, GA., May 1998, and at the Yeast Genetics and Molecular Biology meeting, College Park, MD, August 1998.

7. Gale CA, Gerami-Nejad M, Longtine M, Berman J. A novel localization pattern of septins in Saccharomyces cerevisiae cells expressing the Candida albicans gene INT1. Presented at the Candida and Candidiasis meeting in Charleston, SC, March 1-4, 1999, and at the Society for Pediatric Research meetings in San Francisco, CA, May 1999.

8. Gale CA, Asleson C, Berman J. Expression of Candida albicans INT1 in S. cerevisiae reveals connections between cell cycle control, actin cytoskeleton and septin organization. Presented at the Yeast Cell Biology Meetings, Cold Spring Harbor, August 1999.

9. Gale CA, Gerami-Nejad M, Asleson C, Longtine M, Berman J. Study of Candida albicans INT1 in Saccharomyces cerevisiae reveals connections to the cell cycle and cytoskeleton. Presented at the 99th American Society for Microbiology Meeting in Los Angeles, CA, May 2000.

10. Gale CA, Gerami-Nejad M, McClellan M, Longtine M, Berman J. Candida albicans INT1 interacts with septins in Saccharomyces cerevisiae: implications for a role at the mother-bud neck. Presented at the Yeast Genetics and Molecular Biology meeting in Seattle, WA, August 2000.

11. Gale CA, Gerami-Nejad M, McClellan M, Longtine M, Berman J. Candida albicans Int1 interacts with septins: implications for a role at the mother-bud neck. Presented at the Society for Pediatric Research meetings in Baltimore, MD, April 2001.

12. Gale CA, Gerami-Nejad M, McClellan M, Vandoninck S, Longtine, MS, Berman J. Candida albicans Int1p interacts with septins in yeast and hyphal cells. Presented at the Human Fungal Pathogens Conference in Seefeld, Austria, September 2001.

13. Gale CA, Gerami-Nejad M, Vandoninck S, Warenda A, Konopka J, Berman J. Requirements for Int1p localization in Candida albicans. Presented at the Candida and Candidiasis meeting in Tampa, FL, January 13-17, 2002.

14. Gerami-Nejad M, Berman J, Gale CA. Dynamic localization of Int1p in Candida albicans. Presented at the Society for Pediatric Research meetings in Baltimore, MD, May 2002.

15. Gale CA, Gerami-Nejad M, Hausauer D, Berman J. Linking budding pattern and morphogenesis in Candida albicans. Presented at the Yeast Genetics and Molecular Biology Meeting in Madison, WI, July 2002.

16. Hausauer D. and Gale CA.  Bud2p, a putative GTPase-activating protein, is important for yeast and hyphal morphogenesis in Candida albicans.  Presented at the Society for Pediatric Research meetings in Seattle, WA, May 2003.

17. Hausauer D, Gerami-Nejad M, Berman J, and Gale CA.  Early polarity establishment proteins influence morphogenesis in Candida albicans.  Presented at the Human Fungal Pathogens Conference in Giens, France, September 2003.

18. Hausauer D, Waste M, McClellan M, Masko E, and Gale CA.  The Bud1p-GTPase module influences morphogenesis and regulates septin ring assembly in Candida albicans.  Presented at the Candida and Candidiasis meeting in Austin, TX March 2004.

19. Gerami-Nejad M, Hausauer D, McClellan M, Berman J, and Gale CA.  Cassettes for the PCR-mediated construction of regulatable alleles in Candida albicans. Presented at the Candida and Candidiasis meeting in Austin, TX March 2004.

20. Gerami-Nejad M, Hausauer D, Winning N, and Gale CA.  Identification of domains important for the localization of the Candida albicans morphogenesis protein Int1p.  Presented at the Society for Pediatric Research meetings in San Francisco, CA, May 2004.

Return to top