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Professor
1. Investigation of the impact of dietary copper deficiency during early development on the central nervous system is under study. Characterization of the impact of low intracellular copper on the expression of proteins that function as known cuproenzymes and copper transporters is being investigated to test hypotheses relating biochemistry to behavior.
2. Evaluation of the cuproenzyme peptidylglycine a-amidating monooxygenase and the copper chaperone for superoxide dismutase (CCS) as potential molecular markers to assess nutritional copper status in humans is currently under investigation.
My lab is currently funded by NIH ROI HD-39708 and the USDA NRICGP 2001-00998.
Movie: Ataxia Following perinatal copper deficiency.
Ph.D. Graduate opportunities in my lab may be pursued through University of Minnesota programs in Biochemistry, Molecular Biology and Biophysics, Nutrition, and Toxicology.
Prohaska, J.R., and Brokate, B. (2001) Dietary copper deficiency alters protein levels of rat dopamine alpha-monooxygenase and tyrosine monooxygenase. Exp. Biol. Med. 226: 199-207.
Hamza, I., Faisst, A, Prohaska, J., Chen, J., Gruss, P, and Gitlin, J.D. (2001) The metallochaperone Atox1 plays a critical role in perinatal copper homeostasis. Proc. Natl. Acad. Sci. USA 98: 6848-6852.
Lee, J., Prohaska, J.R., and Thiele, D.J. (2001) Essential role for mammalian copper transporter Ctr1 in copper homeostasis and embryonic development. Proc. Natl. Acad. Sci. USA 98: 6842-6847.
Prohaska, J.R., and Brokate, B. (2001) Lower copper,zinc-superoxide dismutase protein but not mRNA in organs of copper-deficient rats. Arch Biochem. Biophys. 393: 170-176.
Meyer, L.A., Durley, A.P., Prohaska, J.R., and Harris, Z.L. (2001) Copper transport and metabolism are normal in aceruloplasminemic mice. J. Biol. Chem. 276: 36857-36861
Prohaska, J.R., and Brokate, B. (2002) Timing of perinatal copper deficiency in mice influences offspring survival. J.Nutr. 132: 3142-3145.
Hamza, I., Prohaska, J., and Gitlin, J. D. (2003) Essential role for atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase. Proc. Natl. Acad. Sci. USA 100: 1215-1220.
Prohaska, J.R., Geissler, J., Brokate, B., and Broderius, M. (2003) Cu,Zn-superoxide dismutase protein but not mRNA is lower in Cu-deficient mice and mice lacking the metallochaperone CCS. Exp. Biol. Med. 228: 959-966.
Prohaska, J.R., Broderius, M., and Brokate, B (2003) Metallochaperone for Cu,Zn-Superoxide Dismutase (CCS ) Protein but not mRNA is Higher in Organs from Copper-Deficient Mice and Rats. Arch Biochem. Biophys. 417: 227-234.
Gybina, A., and Prohaska, J.R. (2003) Increased rat brain cytochrome c correlates with degree of perinatal copper deficiency rather than apoptosis. J. Nutr. 133: 3361-3368.
Chung, J., Prohaska, J.R., and Wessling-Resnick, M. (2004) Ferroportin-1 mRNA is not upregulated in copper-deficient mice. J. Nutr. 134, 517-521.
Penland, J., and Prohaska, J.R. (2004) Abnormal motor function persists following recovery from perinatal copper deficiency in rats.z J. Nutr. 134, 1984-1988.
West, E.C., and Prohaska, J.R. (2004) Cu,Zn-superoxide dismutase is lower and copper chaperone CCS is higher in erythrocytes of copper-deficient rats and mice. Exp. Biol. Med. 229, 756-764.
Prohaska, J.R., and Gybina, A.A. (2004) Intracellular copper transport in mammals. J. Nutr. 134, 1003-1006.
Prohaska, J.R., Gybina, A.A., Broderius, M., and Brokate, B. (2005) Peptidylglycine alpha-amidating monooxygenase activity and protein are lower in copper-deficient rats and suckling copper-deficient mice. Arch. Biochem. Biophys. 434, 212-220.
Prohaska, J.R., and Gybina, A.A. (2005) Rat brain iron concentration is lower following perinatal copper deficiency. J. Neurochem. 93, 698-705.
Pyatskowit, J.W., and Prohaska, J.R. (2005) L-threo 3,4-dihydroxyphenylserine treatment during mouse perinatal and rat postnatal development does not alter the impact of dietary copper deficiency. Nutr. Neurosci. 8, 173-181.
Kuo, Y-M., Gybina, A.A., Pyatskowit, J.W., Gitschier, J., and Prohaska, J.R. (2006) Copper transport protein (Ctr1) levels in mice are tissue specific and dependent on copper status. J. Nutr. 136, 21-26.
Prohaska, J.R., and Broderius, M. (2006) Plasma peptidylglycine alpha-amidating monooxygenase (PAM) and ceruloplasmin are affected by age and copper-status in rats and mice. Comp. Biochem. Physiol. B. 143, 360-366
Recent Chapters in Books and Symposia Proceedings
Prohaska, J.R. (2000) Neurochemical Alterations Following Perinatal Copper Deficiency in Rodents, in Trace Elements in Man and Animals-10, (A. Favier, R.A. Anderson, and A.M. Rousell, eds.) pp. 909-915, Plenum Press, New York, NY.
Failla, M.L., Johnson, M.A., and Prohaska, J.R. (2001) Copper, in Present Knowledge in Nutrition, 8th Edition (B. Bowman , and R. Russell, eds.) pp.373-383 , ISLI Press, Washington, DC.
Recent Published Abstracts
Prohaska, J.R., and Brokate, B. (2000) Impact of Perinatal Copper (Cu) Deficiency on Rat Dopamine ß-Monooxygenase (DBM). FASEB J. 14, A794.
Prohaska, J.R., and Brokate, B. (2001) Lower Superoxide Dismutase (SOD) Activity and SOD Protein in Copper (Cu) Deficient Rats. FASEB J. 15, A1097.
Prohaska, J.R., and Harris, D.A. (2002) Copper status in mice is not influenced by prion protein expression level. FASEB J. 16, A1097.
Prohaska, J.R., Geissler, J., Broderius, M. and Brokate, B. (2003) Copper-deficient mice and rats have lower Cu,Zn-superoxide dismutase activity and protein but higher metallochaperone CCS levels. FASEB J. 17, A378.
Gybina, A., and Prohaska, J.R. (2004) Elevated Rodent Brain Cytochrome C is Correlated with Degree of Copper Deficiency not Apoptosis. FASEB J. 18, A483.
Prohaska, J.R., and West, E. (2004) Erythrocytes from Copper-Deficient Mice and Rats have Lower Cu,Zn-superoxide dismutase (SOD) but Higher Copper Chaperone (CCS) Levels. FASEB J. 18, A915.
Chung, J., Prohaska J. R., and Wessling-Resnick, M. (2004) Copper deficiency in mice increases hepatic iron stores but does not up-regulate expression of the iron exporter Ferroportin-1. FASEB J. 18, A484.
Pyatskowit, J.W., and Prohaska, J.R. (2005) L-threo 3,4-dihydroxyphenylserine (DOPS) treatment during perinatal development does not alter the impact of dietary copper deficiency in mice. The Toxicologist 84, A279.
Prohaska, J.R., and Gybina, A.A. (2005) Rat brain iron accumulation is dependent on adequate dietary copper FASEB J. 19, A983.