Curriculum Vitae
Daniel B. Leslie, M.D.

EDUCATION AND TRAINING:

1993
B.A., Biochemistry
Dartmouth College, Hanover, NH

1997
M.D., Medicine
University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Camden, NJ

Presently (2007)
Ph.D candidate, Experimental Surgery
University of Minnesota Graduate School, Minneapolis, MN

1997-2000
Intern (PGY 1)
Junior Resident (PGY 2-3)
University of Minnesota Medical School
Department of Surgery, Minneapolis, MN

2000-2004
Research and Surgical Infectious Disease Fellow
University of Minnesota Medical School
Department of Surgery, Minneapolis, MN

2004-2006
Senior Resident (PGY 4)
Chief Resident (PGY 5)
University of Minnesota Medical School
Department of Surgery, Minneapolis, MN

2006-2007
Clinical Instructor
Minimally Invasive Fellow
University of Minnesota Medical School
Department of Surgery, Minneapolis, MN

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CERTIFICATION AND LICENSING:

Minnesota Board of Medical Practice (exp. 4/2007)
DEA registration (exp. 3/2009)
Advanced Trauma Life Support 7/1997 (expired)
Advanced Cardiac Life Support 4/2004 (expired)
Basic Life Support 4/2004 (expired)

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RESEARCH AND TRAINING:

Grant Title: “Native Limulus Anti-Lipopolysaccharide Factor (LALF) – Based Endotoxin Antagonists”
Ethicon-Society of University Surgeons Fellowship Research Award – two year training grant. (2001-2003)
Principal Investigator: Daniel B. Leslie, M.D.

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PROFESSIONAL ACTIVITIES:

1999-2000, Elected representative, Graduate Medical Education Committee (University of Minnesota)
1995-1996, Elected representative, Student Government Association (UMDNJ-RWJMS)
1994-1996, Founder and co-editor, The Weekly RW Johnson medical school  newspaper (UMDNJ-RWJMS)

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HONORS AND AWARDS:

1995, McGraw-Hill Book Award, basic science studies
1992-1993, Richter Memorial Biochemistry Scholarship Grant, undergraduate research funding award, Dartmouth College
1992-1993, Academic Citation-Physical Biochemistry and Physics, Dartmouth College
1993, Rufus Choate Scholar, Dartmouth College
1991-1993, J.H.Barr III scholarship, Dartmouth College
1991, German Book Award, Dartmouth College
1989, National Merit Scholar, Columbine High School

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PUBLICATIONS AND ABSTRACTS:
(* indicates poster presentation)

1. Leslie DB, Lazaron V, Wasiluk KR, Dunn DL. Charge- and sequence-specific characteristics of the endotoxin-binding domain of LALF are critical to bactericidal activity. 36th Annual Meeting of the Association for Academic Surgery, November 7-9, 2002, Boston, MA.

2. Leslie DB, Lazaron V, Wasiluk KR, Dunn DL. Isolation and Characterization Of The Native Complementary DNA Sequence Of Limulus Anti-Lipopolysaccharide Factor. Minnesota Surgical Society Annual Spring Meeting, April 2001, Saint Paul, MN.

3. Lazaron V, Leslie DB, Wasiluk KR, Dunn DL. Internalization and detoxification of lipopolysaccharide (LPS) by anti-LPS antibody is an Fc receptor-mediated process. 62nd Annual Meeting of the Society of University Surgeons, p. 60, February 7-12, 2001, Chicago, IL.

4. Lazaron V, Leslie DB, Wasiluk KR, Dunn DL. Serum transferrin is a cofactor for macrophage activation via cell-surface binding of lipopolysaccharide (endotoxin, LPS). p. 21st Annual Meeting of the Surgical Infection Society, May 3-5, 2001, Snowbird, UT.

5. Lazaron V, Leslie, DB, Wasiluk KR, Dunn DL. Anti-endotoxin antibodies enhance binding and uptake of lipopolysaccharide (LPS) by hepatocytes. 21st Annual Meeting of the Surgical Infection Society, May 3-5, 2001, Snowbird, UT.

6. Lazaron V, Leslie DB, Wasiluk KR, Dunn DL. Inhibition of proinflammatory response to endotoxin by peptides from the extracellular domain of toll-like receptor 4 (TLR4). Surgical Forum LII: 196-198, 2001.

7. Lazaron V, Leslie DB, Wasiluk KR, Dunn DL. Accelerated internalization and detoxification of endotoxin by anti-LPS antibody is an Fc-receptor-mediated process. Surgery 130:192-197, 2001.

8. Lazaron V, Leslie DB, Wasiluk KR, Dunn DL. Localisation and inhibition of pro-inflammatory response to endotoxin by peptides from the extra-cellular domain of Toll-like receptor 4 (TLR4), p. 41, 22d Annual Meeting of the Surgical Infection Society, 15th Annual Meeting of the Surgical Infection Society-Europe, May 2-4, 2002, Madrid, Spain.

9. Lazaron V, Leslie DB, Wasiluk KR, Dunn DL. Inhibition of pro-inflammatory response to endotoxin by peptides from the Toll-like receptor 4 (TLR4-associated protein, MD2) is LBP-dependent. Surgical Forum LIII: 2002.

10. Lazaron V, Leslie DB, Wasiluk KR, Dunn DL. LBP catalyzes the inflammatory response to endotoxin via distinct domains within the LBP-carboxyl domain. 36th Annual Meeting of the Association for Academic Surgery, November 7-9, 2002, Boston, MA.

11. Lazaron V, Leslie DB, Wasiluk KR, Dunn DL. Serum transferrin is a cofactor for macrophage activation via cell-surface binding of lipopolysaccharide (endotoxin, LPS). Ann Surg, submitted.

12. Leslie DB, Vietzen PS, Wasiluk KR, Dunn DL. Cyclization of a peptide based in the LPS-Binding domain of LALF reduces LPS neutralization and bactericidal activity. 23rd Annual meeting of the Surgical Infection Society, p 68, April 10-12, 2003, San Antonio, TX.

13. Leslie DB, Vietzen PS, Lazaron VL, Wasiluk, KR, Dunn DL. Molecular characteristics of the endotoxin-binding domain of LALF. 2003. J Am Coll Surg 197:S33-4.

14. Wasiluk KR, Leslie DB, Vietzen PS, Dunn DL. Enhanced Endotoxin binding of a bioengineered single chain Fv (scFv) monoclonal antibody (mAb). 2003. J Am Coll Surg 197: S41.*

15. Vietzen PS, Leslie DB, Wasiluk KR, Lazaron V, Dunn DL. Addition of positive charge augments the anti-endotoxin properties of LALF-based peptides. 37th Annual Meeting of the Association for Academic Surgery, P43, November 13-15, 2003, Sacramento, CA. JSR 114:286-287, 2003.*

16. Lazaron V, Leslie DB, Wasiluk KR, Vietzen PS, Dunn DL. Molecular anatomy of the pro-inflammatory catalytic activity of lipopolysaccharide-binding protein (LBP). 37th Annual Meeting of the Association for Academic Surgery, P52, November 13-15, 2003, Sacramento, CA. JSR 114:289-290, 2003.

17.  Wasiluk KR, Leslie DB, Vietzen PS, Mayo KH, Dunn DL. Structure/function studies of an endotoxin-neutralizing peptide derived from bactericidal/permeability-increasing protein. 65th Annual Meeting of the Society for University Surgeons, p. 127, February 11-14, 2004, St. Louis, MO.

18. Wasiluk KR, McCulloch KA, Leslie DB, Vietzen PS, Dunn DL. Localization of transcriptional regulatory elements unresponsive to LPS within the TLR4 promoter. 24th Annual Meeting of the Surgical Infection Society, April 29-May 1, 2004, Indianapolis, IN.*

19. Wasiluk KR, Leslie DB, Vietzen PS, Dunn DL. Design of a second-generation novel endotoxin antagonist. 24th Annual Meeting of the Surgical Infection Society, April 29-May 1, 2004, Indianapolis, IN.

20. Vietzen P, Leslie D, Lazaron V, Wasiluk K, Dunn DL. The carboxyl terminus of TIRAP is critical to MyD88-dependent TLR-4 signaling. 2004. J Am Coll Surg 199: S41.

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BOOK CHAPTER:

1. Leslie DB, Dunn DL, Hepatic Abscess. In: Current Surgical Therapy, 8th ed. John L. Cameron. (ed.), Elsevier Mosby, Philadelphia (2004).

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