Dan Kaufman, M.D./Ph.D.

kaufm020@umn.edu

Lab Website:  http://www.med.umn.edu/hot/kaufmanlab/
Education

B.A., Stanford University
M.D., Ph.D. (Immunology), Mayo Medical School and Mayo Graduate School, Rochester, Minn.
Internal medicine residency and fellowship training in hematology, University of Wisconsin-Madison.

Research Interests

Dr. Kaufman is an assistant professor of Medicine in the Division of Hematology, Oncology.

His research interests focus on hematopoietic and endothelial cell development from human and non-human primate embryonic stem (ES) cells. This research uses ES cells to understand the earliest stages of hematopoiesis.

In vitro and in vivo models are used to elucidate both extracellular protein interactions and intracellular genetic regulation that impact these developmental pathways. ES cells offer a unique model to investigate basic developmental biology and may serve as a therapeutic cell source to replace or repair cells or tissues damaged by disease or other degenerative processes.

Selected Publications

• Woll, Petter S., Bartosz Grzywacz, Xinghui Tian, Rebecca Marcus, Michael R. Verneris and Dan S. Kaufman. (2009). Human embryonic stem cells differentiate into a unique population of natural killer cells with highly potent in vivo anti-tumor activity. Blood First Edition Paper, prepublished online April 13, 2009; DOI 10.1182/blood-2008-06-165225 
• Woll, Petter S. J.K. Morris, M.S. Painschab, R.K. Marcus, A.D. Kohn, T.L. Biechele, R.T. Moon, and D.S. Kaufman. (2008). Wnt signaling promotes hemato-endothelial cell development from human embryonic stem cells. Blood, 111: 122-131.
• Tian, Xinghui and Dan S. Kaufman. (2008). Hematopoietic development of human embryonic stem cells in culture. In K. Bunting, ed. Hematopoietic Stem Cell Protocols. Methods in Molecular Medicine. The Humana Press, Inc. Totowa, NJ. 430:119-33.
• Kaufman, Dan S. (2008). The Role of the University in Promoting Human Embryonic Stem Cell Research and Stem Cell Therapies. Minnesota Journal of Law, Science and Technology. 9: 253-256.
• Bera, Tapan K, Ashley Saint Fleur, Duc Ha, Masanori Yamada, Yoomi Lee, Byungkook Lee, Yoonsoo Hahn, Dan S. Kaufman, Martin Pera, and Ira Pastan. (2008). Selective POTE Paralogs on Chromosome 2 are Expressed in Human Embryonic Stem Cells. Stem Cells and Development, 7: 325-332.
• Tian, Xinghui, and Dan S. Kaufman. (2008). Differentiation of embryonic stem cells towards hematopoietic cells: Progress and Pitfalls. Current Opinion in Hematology.15:312-8.
• Cameron, C.M., F. Harding, W.-S. Hu, and  Dan S. Kaufman. (2008). Kinetic activation of a hypoxic response in human embryonic stem cell-derived embryoid bodies. Experimental Biology and Medicine, 233: 1044-1057.
• Martin, Colin H., P. S. Woll, J.-C. Zuniga-Pflucker, and Dan S. Kaufman. (2008). Differences in lymphocyte developmental potential between human embryonic stem cell and umbilical cord blood-derived hematopoietic progenitor cells. Blood. 112: 2730-2737.
• Hill, Katherine and Dan S. Kaufman. (2008). Hematopoietic differentiation of human embryonic stem cells by co-cultivation with stromal layers. In: John Wiley & Sons, Inc. Current Protocols in Stem Cell Biology. Chapter 1:Unit 1F.6.
• Flynn, Catherine and Dan S. Kaufman. (2007). Donor Cell Leukemia: Insight into cancer stem cells and the stem cell niche. Blood 109: 2688-2692.
• Woll, Petter and Dan S. Kaufman. (2007). Derivation of Functional Lymphocytes from Human Embryonic Stem Cells. In: Sullivan, Eggan, and Cowan, eds. Human Embryonic Stem Cells- A Practical Guide. John Wiley & Sons, London, UK. 287-298.
• Andrew Wilber, Jonathan L. Linehan, Xinghui Tian, Petter S. Woll, Julie K. Morris, Lalitha R., Belur, R. Scott McIvor, and Dan S. Kaufman. (2007). Use of the Sleeping Beauty Transposon System for Genetic Engineering of Human Embryonic Stem Cell-derived Hematopoietic Cells. Stem Cells, 25; 2919-2927.
• Tian X., P.S. Woll, J.K. Morris, J.L. Linehan, and Dan S. Kaufman. 2006. Hematopietic Engraftment of Human Embryonic Stem Cell-Derived Blood Cells is Regulated by Host Innate Immunity.    Stem Cells 24:1370-80.
• Cameron, CM, W-S. Hu, and DS. Kaufman. 2006. Improved Development of Human Embryonic Stem Cell-Derived Embryoid Bodies by Stirred Vessel Cultivation.    Biotechnology and Bioengineering. 94: 938-948.
• Hematti, Peiman, Petra Obrtlikova, and Dan S. Kaufman. 2005. Nonhuman Primate Embryonic Stem Cells as a Pre-clinical Model for Hematopoietic and Vascular Repair.    Exp Hematol, 2005, 33: 980-986 .
• Martin, Colin H. and Dan S. Kaufman. 2005. Synergistic use of adult and embryonic stem cells to study human hematopoiesis.  Curr Opin Biotechnol. 16: 510-515.
• Woll, Petter S., Colin H. Martin, Jeffrey S. Miller, and Dan S. Kaufman. 2005. Human embryonic stem cell-derived natural killer cells acquire functional receptors and cytolytic activity.  J. Immunol. 175: 5095-5103 .
• Schwartz, R., J. Linehan, M. Painschab, W.-S. Hu, C. M. Verfaillie, and D. S. Kaufman.2005. Defined conditions for development of functional hepatic cells from human embryonic stem cells.    Stem Cells and Development,14: 643-55.
• Kaufman, Dan S., Rachel L. Lewis, Eric T. Hanson, Robert Auerbach, Johanna Plendl, and James A. Thomson. 2004. Functional Endothelial Cells Derived From Rhesus Monkey Embryonic Stem Cells.    Blood, 103:1325-1332.
• Tian, Xinghui, Julie Morris, Jon Linehan, and Dan S. Kaufman. 2004. Cytokine requirements differ for stroma and embryoid body-mediated mediated hematopoiesis from human embryonic stem cells.    Exp Hematol, 2004, 32:1000-1009.
• Kaufman, Dan S., Eric T. Hanson, Rachel L. Lewis, Robert Auerbach, and James A. Thomson. 2001. Hematopoietic colony-forming cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. USA. 98:10716-10721.