Flow Cytometry FACS Calibur
Genya Gekker, 612-624-0441
Office Hours 9:00 a.m. – 5:00 p.m.
FACS Calibur is a user run, cell analysis device.
After receiving training, it is available for use 24 hrs/day.
Training takes two hours and costs $75 per person and is available by appointment (call 612-624-0441). The training is accepted at the Cancer Center facility.
This is a two laser, four color instrument.
The four most common flourochromes are: FITC, PE, PerCp/PI, and APC. Other colors and markers may be used as long as they match the device specifications.
1) air-cooled argon-ion laser: 488 nm
2) red diode laser: nominally 635 nm
530 nm (FITC), 585 nm (PE/PI), 661 nm (APC), and >650 nm (PerCP) with base unit, >670 nm (APC) with FL4 option
Please see the “Introduction to Flow Cytometry: A Learning Guide” prior to training.