Li-Na Wei, PhD
Professor, Department of Pharmacology
Professor, Department of Pharmacology
Distinguished McKnight University Professor
PhD, Texas Tech University
Dr. Wei's lab is interested in multiple regulatory pathways and underlying mechanisms in differentiation and function of neurons and adipocytes. Two principal signaling pathways are our targets of investigation: a) hormone (vitamin A and fatty acids) signaling pathways that involve nuclear receptors and coregulators to trigger chromatin remodeling, and b) extra-nuclear signaling pathways that regulate post-transcriptional events, specifically, mRNA transport and localized translation.
Our experimental systems include cultures of primary neuron, adipocyte and embryonic stem/embryonal carcinoma cells, as well as genetic animal models. Our experimental methodologies include molecular, biochemical, cellular and genetic methods, as well as heavy uses of mass spec methodologies for proteomic investigation of critical regulatory components in these signaling pathways, such as nuclear receptors, coregulator RIP140, and new RNA binding proteins.
Extending from studies of these basic biological problems, we are also interested in application of these studies in important diseases such as metabolic syndromes and neurological disorders related to, or caused by, abnormal lipid metabolism, mRNA transport and/or translation in neurons.
- Song KY, Choi HS, Hwang CK, Kim CS, Law PY, Wei LN, Loh HH (2009) (OPRM1) Differential use of an in-frame translation initiation codon regulates human mu opioid receptor Cell. Mol. Life Sci. Epub ahead of print July 16, 2009. PMID: 19609488
- Hwang CK, Song KY, Kim CS, Choi HS, Guo XH, Law PY, Wei LN, Loh HH (2009)Epigenetic programming of mu opioid receptor gene in mouse brain is regulated by McCP2 and Brg1 chromatin remodeling factor. J. Cell. Mol. Med. Accepted online October 13, 2008 doi: 10.1111/ j.1582-4934.2008.00535.x. PMID: 19602036
- Song KY, Kim CS, Hwang CK, Choi HS, Law PY, Wei LN, Loh HH (2009) µAUG-mediated translational initiations are responsible for human mu opioid receptor gene expression. J. Cell. Mol. Med. Accepted online March 6, 2009, doi:10.1111/j.1582-4934.2009.00734.x. PMID: 19438807
- Choi HS, Hwang CK, Song KY, Law PY, Wei LN, Loh HH (2009) Poly(C)-binding proteins as transcriptional regulators of gene expression. Biochem. Biophys. Res. Comm. 380:431-436. PMID 19284986; PMCID: PMC2657093 (available on 2010/03/13)
- Gupta P, Ho PC, Ha SG, Lin YW, Wei LN (2009)HDAC3 as a molecular chaperone for shuttling phosphorylated TR2 to PML: a novel deacetylase activity-independent function of HDAC3. PLoS One 4:e4363, 2009. PMID 19204783
- Wu Q, Zhang L, Law PY. Wei LN, Loh HH (2009) Long-term morphine treatment decreases the association of µ-opioid receptor (MOR1) mRNA with polysomes through miRNA23b. Mol. Pharmacol. 75:744-750. PMID 19144786; PMCID: PMC2684920 (available on 2010/04/01)
- Huq MDM, Ha SG, Wei LN (2008) Modulation of retinoic acid receptor alpha activity by lysine methylation in the DNA binding domain. J. Proteomic Res. 7:4538-45. PMID: 18781795
- Wu Q, Law PY, Wei LN, Loh HH (2008)Post-transcriptional regulation of mouse mu-opioid receptor (MOR1) via its 3'UTR-a role of miRNA23b. FASEB J. 22:4085-95. PMID: 18716031; PMCID: PMC2614608 (Available on 12/01/09)
- Kim CS, Hwang CK, Song KY, Choi HS, Kim DK, Law PY, Wei LN, Loh HH (2008) Novel function of neuron-restrictive silencer factor (NRSF) for posttranscriptional regulation. Bioch Bioph Acta 1783:1835-46. PMID: 18657578
- Wei, LN (2008) Epigenetic control of the expression of opioid receptor genes. Epigenetics 3: 119-21. PMID: 18497574
- Choi HS, Song KY, Hwang CK, Kim CS, Law PY, Wei LN, Loh HH (2008) A proteomic approach for identification of single strand DNA binding proteins involved in transcriptional regulation of mouse mu opioid receptor gene. Mol. Cell. Proteom. 7: 1517-29. PMID: 18453338; PMCID: PMC2494908
- Tsai NP, Ho PC, Wei LN (2008)Regulation of stress granule dynamics by Grb7 and FAK signaling pathway. EMBO J. 27: 715-26. PMID: 18273060; PMCID: PMC2265756
- Choi HS, Hwang CK, Kim CS, Song KY, Law PY, Loh HH Wei LN (2008)Transcriptional regulation of mouse mu opioid receptor gene in neuronal cells by poly (ADP-ribose) polymerase-1. J. Cell Mol. Med. 12: 2319-33. PMID 18266974
- Park SW, He Y, Ha SG, Loh HH, Wei LN (2008) 151:1034-1041. Epigenetic regulation of kappa opioid receptor gene in neuronal differentiation. Neuroscience, PMCID: PMC2265776; PMCID: PMC2265776
- Wei LN (2008)Post-translation modifications of orphan nuclear receptors TR2 and TR4 – ligand independent activities. Current Proteomics, 5:211-216.
- Bi J, Tsai NP, Lu HY, Loh HH, Wei LN (2007)Copb1 facilitated axonal transport and translation of kappa opioid receptor mRNA. Proc. Natl. Acad. Sci. USA, 104:13810-13815. PMID: 17698811; PMCID: PMC1959464
- Choi HS, Kim CS, Hwang CK, Song KY, Law PY, Wei LN, Loh HH (2007)Novel function of the poly(C)-binding protein ?CP3 as a transcriptional repressor of the mu opioid receptor gene. FASEB J. 21:3963-3973. PMID: 17625070
- Hwang CK, Song KY, Kim CS, Choi HS, Guo XH, Law PY, Wei LN, Loh HH (2007)Evidence of endogenous mu opioid receptor regulation by epigenetic control of the promoters. Mol. Cell. Biol. 27:4720-4736, 2007. PMID: 17452465; PMCID: PMC1951474
- Tsai NP, Bi J, Wei LN (2007)The adaptor Grb7 links netrin-1 signaling to regulation of mRNA translation. EMBO J. 26:1522-1531. PMID: 17318180; PMCID: PMC1829368
- Song KY, Hwang CK, Kim CS, Choi HS, Law PY, Wei LN, Loh HH (2007)Translational repression of mouse mu opioid receptor expression via leaky scanning. Nucleic Acids Res., 35(5):1501-1513. PMID: 17284463; PMCID: PMC1865057
- Huq MDM, Tsai NP, Lin YP, Higgins LA, Wei LN (2007)Vitamin B6 conjugation to nuclear corepressor RIP140 and its role in gene regulation Nat. Chem. Biol. 3:161-165. PMID: 17277785
- Huq MDM, Tsai NP, Khan SA, Wei LN (2007) Lysine trimethylation of retinoic acid receptor Mol. Cell. Proteomics 6:677-688. PMID: 17205979
- Bi J, Tsai NP, Lin YP, Loh HH, Wei LN (2006)Axonal mRNA transport and localized translational regulation of ?-opioid receptor in primary neurons of dorsal root ganglia, Proc. Natl. Acad. Sci. USA 103(52):19919-19924. PMID: 17167054, PMCID: PMC1750870
- Kim CS, Choi HS, Hwang CK, Song KY, Lee BK, Law PY, Wei LN, Loh HH (2006)Evidence of the neuron-restrictive silencer factor (NRSF) interaction with Sp3 and its synergic repression to the mu opioid receptor (MOR) gene. Nucleic Acids Res. 34(22):6392-6403. PMID: 17130167; PMCID: PMC1702488
- Huq MDM, Gupta P, Tsai NP, White R, Parker MG, Wei LN (2006)Suppression of receptor interacting protein 140 repressive activity by protein arginine methylation. EMBO J 25:5094-5104. PMID: 17053781; PMCID: PMC1630415
- Tsai NP, Bi J, Loh HH, Wei LN (2006)Netrin-1 signaling regulates de novo protein synthesis of ? opioid receptor by facilitating polysomal partition of its mRNA. J Neurosci. 26(38):9743-9749. PMID: 16988045
- Huq MDM, Gupta P, Tsai NP, Wei LN (2006)Modulation of testicular receptor 4 (TR4) activity by MAP-kinase mediated phosphorylation. Mol. Cell. Proteomics 5:2072-2082. PMID: 16887930
- Choi HS, Kim CS, Hwang CK, Song KY, Wang W, Qiu Y, Law PY, Wei LN, Loh HH (2006)The opioid ligand binding of human ?-opioid receptor is modulated by novel splice variants of the receptor. Biochem. Biophys. Res. Comm. 343(4):1132-1140.PMID: 16580639
- Khan SA, Park SW, Huq MDM, Wei LN (2006)Ligand-independent orphan receptor TR2 activation by phosphorylation at the DNA binding domain. Proteomics 6:123-130. PMID: 16317770
- Wei LN, Loh HH (2006)Opioid receptor genes and their regulation. In Handbook in Neuropharmacol.. Springer-Verlag Berlin Heidelberg, pp. 346-355.